Product Name	Trx-Tag
Chinese Name	硫氧还蛋白单克隆抗体
Alias	Thioredoxin; ADF; Surface associated sulphydryl protein; Thioredoxin-1; Thioredoxin 1; Thioredoxin1; TXN protein; ADF; ATL derived factor; DKFZp686B1993; MGC61975; SASP; Surface associated sulphydryl protein; Surface-associated sulphydryl protein; TRDX; TRX 1; TRX; TRX1; TXN; TXN; ATL derived factor; ATL-derived factor; THIO_HUMAN; zgc:92903; Trx-Tag.
Product Type	Tag anti
Research Area	Tumour  Cell biology  Signal transduction  transcriptional regulatory factor
Immunogen Species	Mouse
Clonality	Monoclonal
Clone NO.	7G10
React Species	Recombinant protein
Applications	WB=1:1000-5000 ELISA=1:5000-10000  not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Cellular localization	The nucleus cytoplasmic Secretory protein
Form	Liquid
Concentration	1mg/ml
immunogen	Recombinant human Thioredoxin
Lsotype	IgG
Purification	affinity purified by Protein G
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The protein encoded by this gene acts as a homodimer and is involved in many redox reactions. The encoded protein is active in the reversible S-nitrosylation of cysteines in certain proteins, which is part of the response to intracellular nitric oxide. This protein is found in the cytoplasm. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011] Function: Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity. Subunit: Homodimer; disulfide-linked. Interacts with TXNIP through the redox-active site. Interacts with MAP3K5 and CASP3. In case of infection, interacts with S.typhimurium protein slrP. Interacts with APEX1; the interaction stimulates the FOS/JUN AP-1 DNA-binding activity in a redox-dependent manner. Subcellular Location: Nucleus. Cytoplasm. Secreted. Note=Secreted by a leaderless secretory pathway. Predominantly in the cytoplasm in non irradiated cells. Radiation induces translocation of TRX from the cytoplasm to the nucleus. Similarity: Belongs to the thioredoxin family. Contains 1 thioredoxin domain. SWISS: P10599 Gene ID: 7295 Database links: Entrez Gene: 7295 Human Entrez Gene: 22166 Mouse Entrez Gene: 116484 Rat Omim: 187700 Human SwissProt: P10599 Human SwissProt: P10639 Mouse SwissProt: P11232 Rat Unigene: 435136 Human Unigene: 260618 Mouse Unigene: 29777 Rat
Product Picture	Sample: Lane 1: Trx-tagged fusion protein (32-186) (SL49104P) Overexpression E.coli Lysate at 2 ug Lane 2: Trx-tagged fusion protein (19-213) (SL49106P) Overexpression E.coli Lysate at 2 ug Lane 3: Trx-tagged fusion protein (20-152) (SL49107P) Overexpression E.coli Lysate at 2 ug Primary: Anti-Trx-Tag (SLM-33018M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 30-40 kD Observed band size: 30-40 kD Sample: Lane 1: Recombined Trx/His-tag porcine GM-CSF protein (Aa18-144) at 2 ug Lane 2: Enzyme digested Trx-tag Primary: Anti-Trx-Tag (SLM-33018M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 33/12 kD Observed band size: 33/12 kD Sample: Lane 1: Trx-Tagged fusion protein (full length) Overexpression E.coli Lysate (Cat#: SL33018P) at 4 ug Lane 2: Control (-) E.coli Lysate at 4 ug Primary: Anti-Thioredoxin/Trx (SLM-33018M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 18 kD Observed band size: 18 kD

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