Product Name	T7 tag
Chinese Name	T7 tag（标签）单克隆抗体
Alias	T7-tag; Major capsid protein 10A; MASMTGGQQMG epitope tag; Minor capsid protein 10B; T7 epitope tag.
Product Type	Tag anti
Immunogen Species	Mouse
Clonality	Monoclonal
Clone NO.	3G7
React Species	Recombinant protein
Applications	WB=1:1000-5000 ELISA=1:5000-10000  not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide T7-tag: MASMTGGQQMG
Lsotype	IgG
Purification	affinity purified by Protein G
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The T7 tag is an epitope tag composed of an 11-residue peptide encoded from the leader sequence of the T7 bacteriophage gene10. This gene encodes a T7 major capsid protein whose function is not clear. Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties. The T7 tag is commonly engineered onto the N- or C- terminus of a protein of interest so that the tagged protein can be analyzed and visualized using immunochemical methods. The T7 tag has been used extensively as a general epitope tag in many expression vectors including the pET system that is based on T7 RNA polymerase expression systems. SWISS: N/A Gene ID: N/A
Product Picture	Sample: Lane 1: T7-Tagged Fusion Protein Overexpression E.coli Lysate at 2 ug Lane 2: T7-Tagged Fusion Protein Overexpression E.coli Lysate at 2 ug Primary: Anti-T7-Tag (SLM-33012M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 41 kD Observed band size: 45 kD

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