Product Name	His tag
Chinese Name	聚组氨酸抗体标签单克隆抗体
Alias	HisX6; 6His; His-Tag; 6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; Histidine.
literatures	Specific References  (1)     |     SLM-33004M has been referenced in 1 publications. [IF=4.961] Rong Yue. et al. Study of the Effects of Several SARS-CoV-2 Structural Proteins on Antiviral Immunity. VACCINES-BASEL. 2023 Mar;11(3):524  WB ;  Human.   PubMed:10.3390/vaccines11030524
Product Type	Tag anti
Research Area	Cell biology  immunology
Immunogen Species	Mouse
Clonality	Monoclonal
Clone NO.	6E6
React Species	Recombinant protein
Applications	WB=1:5000-50000 IP=1:200-1000  not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated HHHHHH peptide
Lsotype	IgG
Purification	affinity purified by Protein G
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells. SWISS: N/A Gene ID: N/A 蛋白质纯化的新途径─应用His-Tag抗体检测His-Tag融合蛋白 1） 6xHis与不同的Sepharose纯化填料结合（捆绑），可不受构象的影响，在天然或变形的条件下可进行进一步纯化。 2） 可在温和的洗脱液环境中，其结合、洗涤及洗脱步骤不会对蛋白结构产生影响。 3） 6xHis标签比普通（Tag）要小得多，经纯化后的蛋白可直接进行下游操作。可用于任何表达系统（包括： 细菌，杆状病毒和哺乳动物） 4） 6xHis标签在生理溶液pH下不带电荷，其标签不会干涉重组蛋白的结构和功能， 6xHis不会影响蛋白分泌。 5） 6xHis标签免疫原性差，重组蛋白可以不必除去标签而直接作为抗原进行免疫。
Product Picture	Sample: Lane1: His-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: SL41230P) at 2ug Lane2: His-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: SL41230P) at 2ug Primary: Anti-His tag (SLM-33004M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 41 kD Observed band size: 45 kD Sample: Lane 1: His-Tagged Fusion Protein Overexpression E.coli Lysate (SL41230P) Lane 2: His-Tagged Fusion Protein Overexpression E.coli Lysate (SL41403L) Primary: Anti-His tag (SLM-33004M) at 1/50000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: kDa Observed band size: 45,55 kDa Sample: Lane1:Recombinant JSRV-ENV Protein (His tag) at 100 ng Lane2:Recombinant JSRV-ENV Protein (His tag) at 100 ng Primary: Anti-His tag (SLM-33004M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 35-45 kD Observed band size: 40 kD Sample: His-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: SL41403P) at 4 ug Primary: Anti-His tag (SLM-33004M) at 1/2000 ~ 1/10000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 51 kD Observed band size: 51 kD Sample: Lane 1: His-Tagged Fusion Protein Overexpression E.coli Lysate (SL41230P) at 10ug Primary: Anti-His tag (SLM-33004M) at 1/200000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 41 kDa Observed band size: 45 kDa

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