Product Name	AFP(A4)
Chinese Name	甲胎蛋白单克隆抗体(检测)
Alias	Alpha Fetoprotein; Alpha-fetoprotein; alpha 1 fetoprotein; Alpha-1-fetoprotein; Alpha-fetoglobulin; alpha fetoglobulin; alpha Fetoprotein; Alpha fetoprotein precursor; FETA; HPAFP; FETA_HUMAN.
literatures	Specific References  (6)     |     SLM-1622M has been referenced in 6 publications. [IF=6.641] Zhao J et al. A SERS-based immunoassay for the detection of α-fetoprotein using AuNS@ Ag@ SiO2 core-shell nanostars. Journal of Materials Chemistry C. 2019.  SERS-based sandwich immunoassay ;   PubMed:doi:10.1039/c9tc01890e [IF=6.45] Lin, Dajie, et al. "Cascade signal amplification for electrochemical immunosensing by integrating biobarcode probes, surface-initiated enzymatic polymerization and silver nanoparticle deposition." Biosensors and Bioelectronics (2014).  other ;   PubMed:25460899 [IF=6.277] Zhao CR et al. Highly sensitive and selective electrochemical immunosensors by substrate-enhanced electroless deposition of metal nanoparticles onto three-dimensional Graphene@Ni foams. Science Bulletin.2019.  Other ;   PubMed:doi:10.1016/j.scib.2019.07.015 [IF=5.88] Zhang, Xin, et al. "Spectrum-Based Electrochemiluminescent Immunoassay with Ternary CdZnSe Nanocrystals as Labels." Analytical Chemistry (2016).  other ;   PubMed:27266486 [IF=5.83] Zong, Chen, et al. "Chemiluminescence imaging immunoassay of multiple tumor markers for cancer screening." Analytical Chemistry 84.5 (2012): 2410-2415.  other ;  Human.   PubMed:22320247 [IF=2.475] Huang Y et al. Ag@Au Core⁻Shell Porous Nanocages with Outstanding SERS Activity for Highly Sensitive SERS Immunoassay. Sensors (Basel). 2019 Mar 31;19(7). pii: E1554.  ELISA ;   PubMed:30935114
Research Area	Tumour
Immunogen Species	Mouse
Clonality	Monoclonal
Clone NO.	A4
React Species	Human,
Applications	WB=1:500-2000 ELISA=1:5000-10000  not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	65kDa
Cellular localization	Secretory protein
Form	Liquid
Concentration	1mg/ml
immunogen	AFP protein: full length
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M PBS(pH7.4) with 0.03% Proclin300.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Alpha 1 Fetoprotein is a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. Expression has been documented in human adrenal, liver, ovary, testis, and pancreas. ESTs have been isolated from normal human brain, liver/spleen, embryo and uterus tissue libraries. Function: Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties. Subunit: Dimeric and trimeric forms have been found in addition to the monomeric form. Subcellular Location: Secreted. Tissue Specificity: Plasma. Synthesized by the fetal liver and yolk sac. Similarity: Belongs to the ALB/AFP/VDB family. Contains 3 albumin domains. SWISS: P02771 Gene ID: 174 Database links: Entrez Gene: 174 Human Omim: 104150 Human SwissProt: P02771 Human Unigene: 518808 Human
Product Picture	Sample: Jurkat(Human) Cell Lysate at 30 ug Primary: Anti-AFP(A4) (SLM-1622M) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 65 kD Observed band size: 65 kD Measured by its binding ability in a indirect ELISA. Immobilized Human AFP protein, His Tag (Cat. SL43140P) at 2 μg/mL (100 μL/well) can bind   Mouse Anti-Human AFP Antibody, the EC50 is 20.04 ng/mL.

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