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Product Name Leptin Chinese Name 小鼠抗瘦素单克隆抗体 Alias LEP; Leptin Murine Obesity Homolog; Leptin Precursor Obesity Factor; OB; Obese Protein; Obesity; Obesity factor; Obesity homolog mouse; Obesity Murine Homolog Leptin; OBS; LEP_HUMAN. Research Area Cardiovascular Neurobiology Signal transduction Stem cells Growth factors and hormones The new supersedes the old Immunogen Species Mouse Clonality Monoclonal React Species Rat, (predicted: Human, Mouse, ) Applications ELISA=1:5000-10000 IHC-P=1:400-800 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 16kDa Cellular localization Secretory protein Form Liquid Concentration 1mg/ml immunogen Recombinant human Leptin: 22-167/167 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail This gene encodes a protein that is secreted by white adipocytes, and which plays a major role in the regulation of body weight. This protein, which acts through the leptin receptor, functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing. Mutations in this gene and/or its regulatory regions cause severe obesity, and morbid obesity with hypogonadism. This gene has also been linked to type 2 diabetes mellitus development. [provided by RefSeq, Jul 2008].
Function:
May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass.
Subunit:
Interacts with SIGLEC6.
Subcellular Location:
Secreted (Probable).
DISEASE:
Leptin deficiency (LEPD) [MIM:614962]: A rare disease characterized by low levels of serum leptin, severe hyperphagia and intractable obesity from an early age. Note=The disease is caused by mutations affecting the gene represented in this entry.
Similarity:
Belongs to the leptin family.
SWISS:
P41159
Gene ID:
3952
Database links:Entrez Gene: 3952 Human
Entrez Gene: 16846 Mouse
Omim: 164160 Human
SwissProt: P41159 Human
SwissProt: P41160 Mouse
SwissProt: Q28603 Sheep
Unigene: 194236 Human
Unigene: 277072 Mouse
Unigene: 44444 Rat
Leptin是一种分子量为16kDa的脂肪组织源激素,又称:肥胖蛋白(Obese-protein)是由脂肪细胞分泌的,具有强亲水性,以单体形式存在于血浆中。 Leptin 具有广泛的生物学效应,作用于下丘脑,调节食欲、能量代谢及体重。Leptin 还可能作为脂肪-胰岛内分泌轴的一部分,参与胰岛素分泌的调节。Product Picture Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Leptin) Monoclonal Antibody, Unconjugated (cell supernatant of SLM-10888M 3C2) at overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Leptin) Monoclonal Antibody, Unconjugated (cell supernatant of SLM-10888M 3C2) at overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
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