Mouse Anti-ICAM1/CD54 antibody
BB2; CD54 antigen; CD54; ICAM1; ICAM 1; ICAM-1; Cell surface glycoprotein P3.58; Human rhinovirus receptor; Intercellular adhesion molecule 1; Major group rhinovirus receptor; MALA2; MyD10; P3.58; Surface antigen of activated B cells; ICAM1_HUMAN; MALA-2;
View History [Clear]
Details
Product Name ICAM1/CD54 Chinese Name 细胞间粘附分子-1（CD54）单克隆抗体 Alias BB2; CD54 antigen; CD54; ICAM1; ICAM 1; ICAM-1; Cell surface glycoprotein P3.58; Human rhinovirus receptor; Intercellular adhesion molecule 1; Major group rhinovirus receptor; MALA2; MyD10; P3.58; Surface antigen of activated B cells; ICAM1_HUMAN; MALA-2; MyD10; intercellular adhesion molecule 1 precursor; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; major group rhinovirus receptor. Research Area Cell biology Neurobiology Signal transduction Cell adhesion molecule Cell Surface Molecule glycoprotein Immunogen Species Mouse Clonality Monoclonal Clone NO. 4E5 React Species Human, Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100 IF=1:100-500 （Paraffin sections need antigen repair）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 56kDa Cellular localization The cell membrane Form Liquid Concentration 1mg/ml immunogen Recombinant human ICAM1/CD54: 321-420/532 Lsotype IgG Purification affinity purified by Protein G Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor. [provided by RefSeq, Jul 2008].
Function:
ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.
Subunit:
Homodimer (Probable). Interacts with human herpesvirus 8 MIR2 protein (Probable). Interacts with MUC1 and promotes cell aggregation in epithelial cells. Interacts with ARHGEF26/SGEF. Binds to coxsackievirus A21 capsid proteins and acts as a receptor for this virus.
Subcellular Location:
Membrane; Single-pass type I membrane protein.
Post-translational modifications:
Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.
Similarity:
Belongs to the immunoglobulin superfamily. ICAM family.
ontains 5 Ig-like C2-type (immunoglobulin-like) domains.
SWISS:
P05362
Gene ID:
3383
Database links:
Entrez Gene: 3383 Human
Entrez Gene: 15894 Mouse
Omim: 147840 Human
Unigene: 643447 Human
SwissProt: P05362 Human
SwissProt: P13597 Mouse
Unigene: 435508 Mouse
Unigene: 12 Rat
Product Picture 
Sample:
HUVEC(Human) Cell Lysate at 30 ug
Primary: Anti- ICAM1/CD54 (SLM-10697M) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 100 kD
Sample:
A549(Human) Cell Lysate at 30 ug
MCF-7(Human) Cell Lysate at 30 ug
Primary: Anti-ICAM1'CD54 (SLM-10697M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 90 kD
Paraformaldehyde-fixed, paraffin embedded (Human liver cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ICAM1/CD54) Monoclonal Antibody, Unconjugated (SLM-10697M) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human lung cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ICAM1/CD54) Monoclonal Antibody, Unconjugated (SLM-10697M) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining.
Cartpieces
Totalgoods,subtotals:￥Checkout
References (0)
No References
Bought notes(bought amounts latest0)
No one bought this product
User Comment(Total0User Comment Num)
- No comment
+86 571 56623320
+86 18668110335
