Product Name	BrdU(Proliferation Marker)
Chinese Name	5-溴脱氧尿嘧啶核苷（增殖Maker）单克隆抗体
Alias	Bromodeoxyuridine; Bromodeoxyuridine; 5-Bromo-2′-deoxyuridine; 5-BrdU; Proliferation Marker; 5-Bromo-2-deoxyUridine.
literatures	Specific References  (8)     |     SLM-0917M has been referenced in 8 publications. [IF=8.025] Wei Hao. et al. Ginger polysaccharides relieve ulcerative colitis via maintaining intestinal barrier integrity and gut microbiota modulation. INT J BIOL MACROMOL. 2022 Oct;219:730  IF ;  Mouse.   PubMed:35963344 [IF=5.4] Zhao, Lan, et al. "Acupuncture Improves Cerebral Microenvironment in Mice with Alzheimer’s Disease Treated with Hippocampal Neural Stem Cells."Molecular Neurobiology (2016): 1-11.  IHC-F, IF(ICC) ;  Mouse.   PubMed:27558235 [IF=3.17] Liu, Yang, et al. "Amelioration of Stroke-Induced Neurological Deficiency by Lyophilized Powder of Catapol and Puerarin." International Journal of Biological Sciences 10.4 (2014): 448-456.  IHC-P ;  Mouse.   PubMed:24719562 [IF=2.959] Zhuang M et al. Reelin regulates male mouse reproductive capacity via the sertoli cells.(2018) J. Cell. Biochem. 2018 Oct 18  WB ;  Mouse.   PubMed:30335884 [IF=2.88] Li, Hongwei, et al. "Regular treadmill running improves spatial learning and memory performance in young mice through increased hippocampal neurogenesis and decreased stress." Brain Research (2013).  Mouse.   PubMed:23916669 [IF=2.645] Xiao H et al. Osthole ameliorates cognitive impairments via augmenting neuronal population in APP/PS1 transgenic mice. Neurosci Res . 2020 Apr 14;S0168-0102(20)30008-0.  ICC,WB ;  mouse.   PubMed:32302734 [IF=1.984] Du Y et al. Jieyu Anshen Granule, a Chinese Herbal Formulation, Exerts Effects on Poststroke Depression in Rats. Evidence-Based Complementary and Alternative Medicine, 2020, 1–12.  IHF ;  Rat.   PubMed:doi:10.1155/2020/7469068 [IF=1.89] Wang, N., et al. "Sodium hyaluronate-CNTF gelatinous particles promote axonal growth, neurogenesis and functional recovery after spinal cord injury." Spinal Cord (2014).  IHC-F ;  Rat.   PubMed:24796451
Product Type	Small molecule anti
Research Area	Cell biology  immunology  Developmental biology  Chromatin and nuclear signals  Neurobiology  Drugs and Compounds  Cell type markers
Immunogen Species	Mouse
Clonality	Monoclonal
Clone NO.	1A7
React Species	Human, Rat,
Applications	ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	0.3071kDa
Cellular localization	The nucleus
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated Brdu
Lsotype	IgG
Purification	affinity purified by Protein G
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Proliferation Marker Bromodeoxyuridine (BrdU) is a thymidine analog and is specifically incor-porated into DNA during DNA synthesis. Anti-bromodeoxyuridine monoclonal antibody is used to identify cells that have incorporated BrdU. This immunological detection scheme has several advantages over the use of radioactive thymidine incorporation for identifying cells under-going replication. Labeling and detection can be performed the same day instead of waiting several days, as required for autoradiography of tritium-labeled cells, and the necessity of using multiple specimens for obtaining the optimal exposure time is eliminated. In addition, anti-bromodeoxyuridine staining with flow cytometric analysis allows multiple parameters to be evaluated simultaneously. Anti-bromodeoxyuridine monoclonal antibody has been used for identi-fying proliferating cells in blood (Campana et al., 1988), tissues (Schutte et al., 1987; Hayashi, et al., 1988), tumors (Hoshino et al., 1986; Morstyn et al., 1983), as well as for determining plasma cell labeling indices (Greipp et al., 1985). Subcellular Location: Nuclear. SWISS: N/A CAS: 59-14-3 增殖Maker BrdU（溴化脱氧尿嘧啶核苷）可以在体内和体外掺入到处于S期的细胞所合成的DNA链中。此抗体可以与掺入到任何种属细胞中的BrdU反应，与碘脱氧尿嘧啶有React Species，标记掺有BrdU的S期细胞，主要用于研究各种不同的因素对正常/Tumour组织的细胞增殖及动力学的研究。 用FITC标记的抗BrdU IgG可用于流式细胞术定量检测增值细胞。
Product Picture	Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (BrdU(Proliferation Marker) ) Monoclonal Antibody, Unconjugated (SLM-0917M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (BrdU(Proliferation Marker) ) Monoclonal Antibody, Unconjugated (SLM-0917M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Mouse Anti-BrdU(A7) Monoclonal Antibody, Unconjugated(SLM-0917M) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0024) and DAB(C-0010) staining Tissue/cell: rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: The primary antibody was Mouse Anti-BrdU(A7) Monoclonal Antibody, FITC conjugated (SLM-0917M-FITC) 1:200, 40 minutes at 37°C; The secondary antibody was Rabbit Anti-Tubulin Beta, Cy3 conjugated(SL4511R-Cy3) 1:200, 40 minutes at 37°C.

Cartpieces

• 
• 9

  9Delete

• 
• 5

  5Delete

• 
• S

  SDelete

• 
• M

  MDelete

• 
• +

  +Delete

• 
• 4

  4Delete

• Delete

• Delete

• 
• 1

  1Delete

• 
• 1

  1Delete

• 
• $

  $Delete

• 
• 0

  0Delete

• 
• 4

  4Delete

• 
• $

  $Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 1

  1Delete

• 
• B

  BDelete

• 
• B

  BDelete

• 

• Delete

• 
• i

  iDelete

• 
• i

  iDelete

• Delete

• 
• 1

  1Delete

• Delete

• 
• 1

  1Delete

• 
• 1

  1Delete

• 
• 0

  0Delete

• Delete

• 
• 2

  2Delete

• 
• 1

  1Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 1

  1Delete

• 
• 1

  1Delete

• Delete

• 
• -

  -Delete

• 
• -

  -Delete

• 
• 0

  0Delete

• Delete

• 
• t

  tDelete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• f

  fDelete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• 
• 4

  4Delete

• 
• $

  $Delete

• Delete

• 
• 0

  0Delete

• Delete

• Delete

• Delete

• 
• M

  MDelete

• 
• 5

  5Delete

• 
• A

  ADelete

Totalgoods,subtotals:￥Checkout