Product Name	ELYS
Chinese Name	转录因子ELYS蛋白抗体
Alias	ahctf1; AT hook containing transcription factor 1; DKFZp434N093; ELYS; AT-hook-containing transcription factor 1; ELYS transcription factor like protein TMBS62; ELYS_HUMAN; Embryonic large molecule derived from yolk sac; MST108; MSTP108; Protein ELYS; Protein MEL-28; Putative AT-hook-containing transcription factor 1; TMBS62.
Research Area	Cardiovascular  Stem cells  Epigenetics
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Mouse,  (predicted: Human, Rat, Pig, Cow, Horse, Sheep, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	252kDa
Cellular localization	The nucleus cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human ELYS: 451-550/2266
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	ELYS is a protein of the nuclear matrix that contains one AT hook DNA-binding domain. ELYS is a dual nucleoporin/kinetochore protein required for nuclear pore assembly and proper cell division. Nuclear pores span the nuclear envelope and act as gated aqueous channels to regulate the transport of macromolecules between the nucleus and cytoplasm, from individual proteins and RNAs to entire viral genomes. The A.T. hook near the C-terminus of likely indicates ELYS can function as a transcription factor which may play a specific role in hematopoietic tissues. It also may play a role in early embryo development, as ELYS is critical for the survival of inner cells of the blastocyst. Function: Required for the assembly of a functional nuclear pore complex (NPC) on the surface of chromosomes as nuclei form at the end of mitosis. May initiate NPC assembly by binding to chromatin and recruiting the Nup107-160 subcomplex of the NPC. Also required for the localization of the Nup107-160 subcomplex of the NPC to the kinetochore during mitosis and for the completion of cytokinesis. Subunit: Associates with the Nup107-160 subcomplex of the NPC. Subcellular Location: Cytoplasm. Nucleus matrix. Chromosome; centromere; kinetochore. Nucleus; nucleoplasm. Nucleus; nuclear pore complex. Localizes to the nuclear pore complex (NPC) throughout interphase. Localizes to the kinetochore from prophase, and this appears to require the Nup107-160 subcomplex of the NPC. Localizes to the periphery of chromatin from late anaphase. Similarity: Belongs to the ELYS family. Contains 1 A.T hook DNA-binding domain. SWISS: Q8WYP5 Gene ID: 25909 Database links: Entrez Gene: 25909 Human Omim: 610853 Human SwissProt: Q8IZA4 Human SwissProt: Q8WYP5 Human Unigene: 300887 Human
Product Picture	Sample: Spinal cord (Mouse) Lysate at 40 ug Primary: Anti- ELYS (SL9880R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 252 kD Observed band size: 252 kD Paraformaldehyde-fixed, paraffin embedded (mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ELYS) Polyclonal Antibody, Unconjugated (SL9880R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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