Product Name	Rap2A
Chinese Name	Rap2A抗体
Alias	KREV; RAP2A, member of RAS oncogene family; Ras-related protein Rap-2a; RbBP 30; RbBP30; RAP2A_HUMAN.
Research Area	Cell biology  immunology
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Rat,  (predicted: Human, Mouse, Chicken, Dog, Cow, Horse, Rabbit, Sheep, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	21kDa
Cellular localization	cytoplasmic The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human Rap2A: 121-183/183
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Ras oncogenes encode GTP-binding proteins that are capable of transforming immortalized cells in culture. Two Ras-related human genes, designated RAP1A and RAP1B, encode 95% homologous proteins (namely Rap 1A and Rap 1B) that share a similar C-terminal Cys-Ali-Ali-Xaa sequence with Ras proteins and are ubiquitously expressed in mammalian tissues. The putative “effector” domain of Ras proteins, whose integrity is required for cell transformation as well as interaction with the putative effector protein GAP, is conserved in both Rap 1 proteins. Rap 1A is thought to interfere with Ras effector function by binding to Ras GAP in a GTP-dependent manner without affecting Rap 1A GTPase activity. Rap 2, another Ras-related protein, shares 60% identity with Rap 1A and exhibits a carboxy terminal CAAX motif and two upstream cysteines similar to those of the H-Ras, K-Ras and N-Ras proteins. In contrast with Rap 1A and Rap 1B, overexpression of Rap 2 does not interfere with the Ras signaling pathway. Function: Small GTP-binding protein which cycles between a GDP-bound inactive and a GTP-bound active form. In its active form interacts with and regulates several effectors including MAP4K4, MINK1 and TNIK. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. More generally, it is part of several signaling cascades and may regulate cytoskeletal rearrangements, cell migration, cell adhesion and cell spreading. Subunit: Interacts (GTP-bound form) with RUNDC3A. Interacts with RGS14; the interaction is GTP-dependent (By similarity). Interacts with PLCE1. Interacts with ARHGAP29, SGSM1, SGSM2 and SGSM3. Interacts (GTP-bound form preferentially) with TNIK (via the CNH domain); the interaction is direct and recruits RAP2A to the E3 ubiquitin ligase NEDD4. Interacts with MINK1. Interacts (GTP-bound form preferentially) with MAP4K4. Interacts with cytoskeletal actin. Subcellular Location: Recycling endosome membrane; Lipid-anchor; Cytoplasmic side. Note=May also localize to the Golgi (PubMed:7962206) and the gelatinase-containing granules of neutrophils (PubMed:8391995). Post-translational modifications: Ubiquitinated; undergoes 'Lys-63' monoubiquitination and diubiquitination by NEDD4. Multiple lysine residues are probably modified. Ubiquitination requires TNIK, prevents interaction with effectors and inactivates RAP2A. Palmitoylated. Palmitoylation is required for association with recycling endosome membranes and activation of TNIK (By similarity). Similarity: Belongs to the small GTPase superfamily. Ras family. SWISS: P10114 Gene ID: 5911 Database links: Entrez Gene: 5911 Human Omim: 179540 Human SwissProt: P10114 Human
Product Picture	Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Rap2A) Polyclonal Antibody, Unconjugated (SL9872R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

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