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Product Name XTP4 Chinese Name 乙型肝炎病毒X蛋白反转录蛋白4抗体 Alias C17orf37; C35 antibody chromosome 17 open reading frame 37; HBV X transactivated gene 4 protein; HBV X-transactivated gene 4 protein; HBV XAg transactivated protein 4; HBV XAg-transactivated protein 4; MGC14832; Mien1; MIEN1_HUMAN; Migration and invasion enhancer 1; ORB3; Protein C35; RDX12. Research Area Cell biology immunology transcriptional regulatory factor Bacteria and viruses Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, (predicted: Human, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep, ) Applications ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=2ug/Test ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 12kDa Cellular localization cytoplasmic The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human XTP4: 33-130/616 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Increases cell migration by inducing filopodia formation at the leading edge of migrating cells. Plays a role in regulation of apoptosis, possibly through control of CASP3. May be involved in a redox-related process.
Function:
Increases cell migration by inducing filopodia formation at the leading edge of migrating cells. Plays a role in regulation of apoptosis, possibly through control of CASP3. May be involved in a redox-related process.
Subunit:
Interacts with GPX1 (By similarity).
Subcellular Location:
Cellular localizationCytoplasm; cytosol. Cell membrane. Concentrates at the leading edge of migrating cells. Localizes outside membrane raft regions.
Tissue Specificity:
Among normal tissues, present only in Leydig cells. Strongly up-regulated in breast cancers and in brain cancer distant metastasis (at protein level). Up-regulated in prostate cancer cells and in the higher grades of prostate adenocarcinoma (at protein level).
Post-translational modifications:
Isoprenylation facilitates association with the plasma membrane and enhances the migratory phenotype of cells by inducing increased filopodia formation.
Similarity:
Belongs to the SelWTH family.
SWISS:
Q9BRT3
Gene ID:
84299
Database links:Entrez Gene: 84299 Human
Entrez Gene: 103742 Mouse
Omim: 611802 Human
SwissProt: Q9BRT3 Human
SwissProt: Q9CQ86 Mouse
Unigene: 333526 Human
Unigene: 259614 Mouse
Unigene: 143893 Rat
Product Picture Blank control:Mouse kidney.
Primary Antibody (green line): Rabbit Anti-XTP4 antibody (SL9636R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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