Product Name	PINX-1
Chinese Name	端粒酶抑制因子PinX-1抗体
Alias	67-11-3 protein; FLJ20565; Hepatocellular carcinoma-related putative tumor suppressor; Liver-related putative tumor suppressor; LPTL; LPTS; MGC8850; PIN2 interacting protein 1; Pin2-interacting protein X1; PIN2/TERF1-interacting telomerase inhibitor 1; PINX1; PINX1_HUMAN; Protein 67-11-3; TRF1-interacting protein 1.
Research Area	Tumour  Cell biology  transcriptional regulatory factor
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Mouse,  (predicted: Human, Rat, Pig, Cow, Horse, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=2ug/Test ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	37kDa
Cellular localization	The nucleus
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human PINX-1: 65-160/328
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	PINX1 is a ubiquitously expressed protein that localizes to nucleoli and telomere speckles. PINX1 contains one G-patch domain and one telomeric inhibiting domain (TID) at its C-terminus. PINX1 interacts with the telomere protein TRF1 and the telomerase reverse transcriptase TERT. The TID domain of PINX1 specifically interacts with TERT and functions to inhibit its activity, thus participating in the regulation of telomerase activity. Overexpression of PINX1 leads to shortened telomeres, further supporting an inhibitory role of PINX1 on telomerase activity. The depletion of PINX1 significantly increases telomerase activity and may lead to tumorigenicity of cancer cells. This suggests that PINX1 acts as a tumor suppressor and can inhibit cell proliferation. In addition, PINX1 is involved in nucleolar RNA maturation. Function: Microtubule-binding protein essential for faithful chromosome segregation. Mediates TRF1 and TERT accumulation in nucleolus and enhances TRF1 binding to telomeres. Inhibits telomerase activity. May inhibit cell proliferation and act as tumor suppressor. Subunit: Interacts with MCRS1, TERT, TERF1, NCL/nucleolin, and the telomerase RNA. Subcellular Location: Nucleus. Tissue Specificity: Ubiquitous; expressed at low levels. Similarity: Belongs to the PINX1 family. Contains 1 G-patch domain. SWISS: Q96BK5 Gene ID: 54984 Database links: Entrez Gene: 54984 Human Entrez Gene: 72400 Mouse Entrez Gene: 305963 Rat Omim: 606505 Human SwissProt: Q548A5 Human SwissProt: Q6QWG9 Human SwissProt: Q96BK5 Human SwissProt: Q9CZX5 Mouse SwissProt: A4L691 Rat Unigene: 490991 Human Unigene: 583894 Human Unigene: 379214 Mouse Unigene: 15365 Rat
Product Picture	Tissue/cell: mouse kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-PINX-1 Polyclonal Antibody, Unconjugated(SL9594R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Blank control: Mouse spleen. Primary Antibody (green line): Rabbit Anti-PINX-1 antibody (SL9594R) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed

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