Product Name	SHARPIN
Chinese Name	线性Ubiquitin链相关蛋白SHARPIN抗体
Alias	hSIPL1; Shank associated RH domain interacting protein; SHANK associated RH domain interactor; Shank interacting protein like 1; Shank-associated RH domain-interacting protein; Shank-interacting protein-like 1; Sharpin; SHRPN_HUMAN; SIPL1; DKFZp434N1923.
Research Area	Tumour  Cell biology  Ubiquitin
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Mouse,  (predicted: Human, Rat, Dog, )
Applications	ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	40kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human SHARPIN: 271-387/387
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	SHARPIN is a 387 amino acid protein that localizes to the cytoplasm and contains one RanBP2-type zinc finger. Expressed at high levels in placenta and skeletal muscle and present at lower levels in colon, brain, heart, liver, kidney, lung, thymus and small intestine, SHARPIN interacts with Shank 1 and is thought to play a role in the control of inflammatory responses and in the overall development of the immune system. SHARPIN exists as three alternatively spliced isoforms and shares 73% sequence identity with its mouse counterpart, suggesting a conserved role between species. The gene encoding SHARPIN maps to human chromosome 8, which consists of nearly 146 million base pairs, houses more than 800 genes and is associated with a variety of diseases and malignancies. Function: May have a role in normal immune development and control of inflammation. Subunit: Monomer and homodimer. Interacts with SHANK1, EYA1 and EYA2 (By similarity). Component of the LUBAC complex (linear ubiquitin chain assembly complex) which consists of SHARPIN, RBCK1 and RNF31. LUBAC has a MW of approximative 600 kDa suggesting a heteromultimeric assembly of its subunits. Associates with the TNF-R1 signaling complex (TNF-RSC) in a stimulation-dependent manner. Subcellular Location: Cytoplasm. Enriched at synaptic sites in mature neurons where it colocalizes with SHANK1. Tissue Specificity: Highly expressed in skeletal muscle and placenta and at lower levels in brain, heart, colon without mucosa, thymus, spleen, kidney, liver, small intestine, lung and peripheral blood leukocytes. Similarity: Contains 1 RanBP2-type zinc finger. SWISS: Q9H0F6 Gene ID: 81858 Database links: Entrez Gene: 81858 Human Omim: 611885 Human SwissProt: Q9H0F6 Human Unigene: 529755 Human
Product Picture	Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SHARPIN) Polyclonal Antibody, Unconjugated (SL9581R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.

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