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Product Name KRCC1 Chinese Name 含赖氨酸卷曲螺旋蛋白1抗体 Alias CHBP2; cryptogenic hepatitis binding protein; Cryptogenic hepatitis-binding protein 2; KRCC1; KRCC1_HUMAN; lysine rich coiled coil 1; Lysine-rich coiled-coil protein 1. Research Area Cell biology immunology Developmental biology Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, (predicted: Human, Rat, Cow, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 31kDa Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human KRCC1: 161-259/259 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail KRCC1 is a 259 amino acid protein that is encoded by a gene located on human chromosome 2p11.2. Consisting of 237 million bases, chromosome 2 is the second largest human chromosome and encodes over 1,400 genes. A number of genetic diseases are linked to genes on chromosome 2. Harlequin icthyosis, a rare and morbid skin deformity, is associated with mutations in the ABCA12 gene. The lipid metabolic disorder sitosterolemia is associated with ABCG5 and ABCG8. An extremely rare recessive genetic disorder, Alstr鰉 syndrome, is due to mutations in the ALMS1 gene. Interestingly, chromosome 2 contains what appears to be a vestigial second centromere and vestigial telomeres which gives credence to the hypothesis that human chromosome 2 is the result of an ancient fusion of two ancestral chromosomes seen in modern form today in apes.
SWISS:
Q9NPI7
Gene ID:
51315
Database links:Entrez Gene: 51315 Human
SwissProt: Q9NPI7 Human
Unigene: 469254 Human
Product Picture Sample:
Kidney (Mouse) Lysate at 40 ug
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti-KRCC1 (SL9560R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 31 kD
Observed band size: 31 kD
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KRCC1) Polyclonal Antibody, Unconjugated (SL9560R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.Paraformaldehyde-fixed, paraffin embedded (Mouse Kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KRCC1) Polyclonal Antibody, Unconjugated (bs9560R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.Paraformaldehyde-fixed, paraffin embedded (Mouse Brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KRCC1) Polyclonal Antibody, Unconjugated (bs9560R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.
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