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Product Name C9orf86 Chinese Name 9号染色体开放阅读框86抗体 Alias Chromosome 9 open reading frame 86; FLJ10101; Hypothetical protein LOC55684; KIAA1984; Parf; Partner of ARF; pp8875; Putative GTP binding protein Parf; Rab like GTP binding protein 1; Rab like GTP binding protein 1C; Rab like protein 1; RBEL1; RP11-216L13.9. Research Area Tumour Cell biology Epigenetics Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, Rat, Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 80kDa Cellular localization The nucleus cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human C9orf86: 631-729/729 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail C9orf86 (Partner of ARF;PARF) binds p19(ARF), but not the p16(INK4A) isoform of CDKN2A. Knockdown of C9orf86 by RNA interference reduced the growth inhibitory activity of p19(ARF) and enhanced the rate of cell proliferation.
Function:
May enhance cellular proliferation. May reduce growth inhibitory activity of CDKN2A.
Subcellular Location:
Cytoplasmic and Nuclear. Note=Predominantly cytoplasmic. Proposed to shuttle between cytoplasm and nucleus.
Post-translational modifications:
Isoform 1 is O-glycosylated, while other isoforms are not.
Similarity:
Belongs to the small GTPase superfamily. Rab family.
SWISS:
Q3YEC7
Gene ID:
55684
Database links:Entrez Gene: 55684 Human
Entrez Gene: 227624 Mouse
Omim: 610615 Human
SwissProt: Q3YEC7 Human
SwissProt: Q5U3K5 Mouse
Unigene: 370555 Human
Unigene: 29600 Mouse
Unigene: 443064 Mouse
Unigene: 17709 Rat
Product Picture Sample:
Stomach (Mouse) Lysate at 40 ug
Primary: Anti- C9orf86 (SL9545R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 80 kD
Observed band size: 80 kD
Paraformaldehyde-fixed, paraffin embedded (mouse stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (C9orf86) Polyclonal Antibody, Unconjugated (SL9545R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (C9orf86) Polyclonal Antibody, Unconjugated (SL9545R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (C9orf86) Polyclonal Antibody, Unconjugated (SL9545R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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