Product Name	HAND1
Chinese Name	心脏和神经嵴衍生蛋白1抗体
Alias	autonomic nervous system and neural crest derivatives-expressed protein 1; heart; Basic helix loop helix transcription factor HAND1; Class A basic helix-loop-helix protein 27; Extraembryonic tissues; Extraembryonic tissues heart autonomic nervous system and neural crest derivatives expressed protein 1; HAND 1; HAND1; HAND-1; HAND1_HUMAN; Heart and neural crest derivatives expressed 1; Heart and neural crest derivatives expressed protein 1; Heart-and neural crest derivatives-expressed protein 1; Hxt; Thing 1; Thing1.
literatures	Specific References  (2)     |     SL9459R has been referenced in 2 publications. [IF=12.153] Tang, Chao. et al. RGS2 promotes estradiol biosynthesis by trophoblasts during human pregnancy. EXP MOL MED. 2023 Jan;:1-13  WB ;  Human.   PubMed:36653442 [IF=4.275] Haibin Zhu. et al. Human HAND1 Inhibits the Conversion of Cholesterol to Steroids in Trophoblasts. J Genet Genomics. 2021 Aug;:  IP ;  Human.   PubMed:34391879
Research Area	Cardiovascular  Developmental biology  Stem cells  transcriptional regulatory factor  Epigenetics
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Dog,  (predicted: Rat, Chicken, Pig, Cow, Rabbit, Sheep, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	24kDa
Cellular localization	The nucleus
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human HAND1: 101-200/215
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Transcription factor that plays an essential role in both trophoblast-giant cells differentiation and in cardiac morphogenesis. In the adult, could be required for ongoing expression of cardiac-specific genes.dHAND (for deciduum, heart, autonomic nervous system and neural crest derivatives; also designated HAND2) and eHAND (also designated HAND1, HXT or Thing1) are members of a subclass of basic-helix-loop-helix transcription factors that are involved in cardiac development. dHAND and eHAND are expressed in the heart after cardiac looping, and they participate in left-right cardiac asymmetry. dHAND is expressed predominantly on the right side of the looped heart tube and in the pulmonary ventricle, where it activates transcription of various genes, including Ufd1 (for ubiquitin fusion degradation) and Cdc45. In addition, dHAND is expressed in sympathetic neurons and chromafin cells throughout embryonic and fetal development and mediates neural crest development. eHAND expression is primarily observed on the left side and in the systemic ventricle, suggesting that these proteins are involved in the development of segments of the heart tube, which give rise to specific heart chambers during cardiogenesis. Function: Transcription factor that plays an essential role in both trophoblast-giant cells differentiation and in cardiac morphogenesis. In the adult, could be required for ongoing expression of cardiac-specific genes. Binds the DNA sequence 5'-NRTCTG-3' (non-canonical E-box) (By similarity). Subunit: Efficient DNA binding requires dimerization with another bHLH protein. Forms homodimers and heterodimers with TCF3 gene products E12 and E47, HAND2 and HEY1, HEY2 and HEYL (hairy-related transcription factors). Interacts with MDFIC (By similarity). Subcellular Location: Nucleus. Tissue Specificity: Heart. Post-translational modifications: Phosphorylation by PLK4 disrupts the interaction with MDFIC and leads to tranlocation into the nucleoplasm, allowing dimerization and transcription factor activity (By similarity). Similarity: Contains 1 bHLH (basic helix-loop-helix) domain. SWISS: O96004 Gene ID: 9421 Database links: Entrez Gene: 9421 Human Entrez Gene: 59112 Rat Omim: 602406 Human SwissProt: O96004 Human SwissProt: P57100 Rabbit SwissProt: P97832 Rat SwissProt: Q28555 Sheep Unigene: 152531 Human Unigene: 224598 Rat Unigene: 39340 Rat
Product Picture	Sample: HL-60(Human) Cell Lysate at 30 ug Primary: Anti- HAND1 (SL9459R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 24 kD Observed band size: 25 kD Sample: Placenta (Mouse) Lysate at 40 ug HL-60 Cell (Human) Lysate at 40 ug Primary: Anti-HAND1 (SL9459R) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (SL0295G-HRP) at 1/5000 dilution Predicted band size: 24 kD Observed band size: 26 kD

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Totalgoods,subtotals:￥Checkout