Product Name	Hexokinase II
Chinese Name	己糖激酶2抗体
Alias	Hexokinase 2; Hexokinase 2 muscle; Hexokinase type II; Hexokinase-2; HK 2; HK II; HK2; HKII; HxK 2; HxK2; HXK2_HUMAN; Muscle form hexokinase.
literatures	Specific References  (2)     |     SL9455R has been referenced in 2 publications. [IF=9.314] Zhou et al. Melatonin protects cardiac microvasculature against ischemia/reperfusion injury via suppression of mitochondrial fission-VDAC1-HK2-mPTP-mitophagy axis. (2017) J.Pineal.Re. 63  WB,IF(ICC) ;  Mouse.   PubMed:28398674 [IF=2.886] Yan Liu. et al. CircTTBK2 Contributes to the Progression of Glioma Through Regulating miR-145-5p/CPEB4 Axis. Cancer Manag Res. 2020; 12: 8183–8195  WB ;  Human.   PubMed:32982415
Research Area	Tumour  Cardiovascular  Chromatin and nuclear signals  Signal transduction  transcriptional regulatory factor  The new supersedes the old  Mitochondrion
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse,  (predicted: Rat, Dog, Pig, Cow, Rabbit, Sheep, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	102kDa
Cellular localization	cytoplasmic The cell membrane Mitochondrion
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human Hexokinase II/HxK2: 101-200/917
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The hexokinases utilize Mg-ATP as a phosphoryl donor to catalyze the first step of intracellular glucose metabolism, the conversion of glucose to glucose-6-phosphate. Four hexokinase isoenzymes have been identified, including hexokinase I (HXK I), hexokinase II (HXK II), hexokinase III (HXK III) and hexokinase IV (HXK IV, also designated glucokinase or GCK). Hexokinases I-III each contain an N-terminal cluster of hydrophobic amino acids. Glucokinase lacks the N-terminal hydrophobic cluster. The hydrophobic cluster is thought to be necessary for membrane binding. This is substantiated by the finding that glucokinase has lower affinity for glucose than do the other hexokinases. HXK I has been shown to be expressed in brain, kidney and heart tissues as well as in hepatoma cell lines. HXK II is involved in the uptake and utilization of glucose by adipose and skeletal tissues. Of the hexokinases, HXK III has the highest affinity for glucose. Glucokinase is expressed in pancreatic beta cells where it functions as a glucose sensor, determining the “set point” for insulin secretion. Subunit: Monomer (By similarity). Subcellular Location: Mitochondrion outer membrane. Its hydrophobic N-terminal sequence may be involved in membrane binding. Tissue Specificity: Predominant hexokinase isozyme expressed in insulin-responsive tissues such as skeletal muscle. Similarity: Belongs to the hexokinase family. SWISS: P52789 Gene ID: 3099 Database links: Entrez Gene: 3099 Human Omim: 601125 Human SwissProt: P52789 Human Unigene: 406266 Human Unigene: 591588 Human
Product Picture	Sample: Heart (Mouse) Lysate at 40 ug Muscle (Mouse) Lysate at 40 ug Primary: Anti-Hexokinase II (SL9455R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 102 kD Observed band size: 102 kD Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Hexokinase 2) Polyclonal Antibody, Unconjugated (SL9455R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Mouse stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Hexokinase 2) Polyclonal Antibody, Unconjugated (SL9455R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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