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Product Name SMURF1 Chinese Name Smad蛋白E3Ubiquitin连接酶1抗体 Alias Smad ubiquitination regulatory factor 1; Smad ubiquitination regulatory factor 1; Smad specific E3 ubiquitin ligase 1; E3 ubiquitin-protein ligase SMURF1; hSMURF1; Smad specific E3 ubiquitin ligase 1; SMAD specific E3 ubiquitin protein ligase 1; Smad ubiquitination regulatory factor 1; SMAD-specific E3 ubiquitin-protein ligase 1; SMUF1_HUMAN; SMURF 1; smurf1. literatures Specific References (1) | SL9391R has been referenced in 1 publications.[IF=4.534] Weng S et al. Improvement of muscular atrophy by AAV–SaCas9-mediated myostatin gene editing in aged mice. Cancer Gene Ther. 2020 Dec;27(12):960-975. WB ; Mouse.Research Area Cell biology Cyclin Immunogen Species Rabbit Clonality Polyclonal React Species Rat, (predicted: Human, Mouse, Chicken, Dog, Pig, Cow, Horse, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 83kDa Cellular localization cytoplasmic The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human SMURF1: 65-170/757 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail E3 ubiquitin-protein ligase that acts as a negative regulator of BMP signaling pathway. Acts by mediating ubiquitination and degradation of SMAD1 and SMAD5, 2 receptor-regulated SMADs specific for the BMP pathway. Promotes ubiquitination and subsequent proteasomal degradation of TRAF family members.
Subcellular Location:
Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side.
Similarity:
Contains 1 C2 domain.
Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
Contains 2 WW domains.
SWISS:
Q9HCE7
Gene ID:
75154
Database links:Entrez Gene: 57154 Human
Entrez Gene: 75788 Mouse
Omim: 605568 Human
SwissProt: Q9HCE7 Human
SwissProt: Q9CUN6 Mouse
Unigene: 189329 Human
Unigene: 27735 Mouse
Product Picture Paraformaldehyde-fixed, paraffin embedded (rat stomach tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SMURF1) Polyclonal Antibody, Unconjugated (SL9391R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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