Product Name	RNF61/MKRN1
Chinese Name	Ring finger protein61抗体
Alias	RING finger protein 61; E3 ubiquitin-protein ligase makorin-1; Makorin; Makorin ring finger protein 1; MKRN 1; MKRN-1; mkrn1; MKRN1_HUMAN; Ring finger protein 1; RNF61.
Research Area	Cell biology  Cyclin  Epigenetics
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Rat,  (predicted: Human, Mouse, Dog, Pig, Cow, Horse, Rabbit, Sheep, )
Applications	ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	53kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human MKRN1/RNF61: 261-360/482
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. These substrates include FILIP1, p53/TP53, CDKN1A and TERT. Keeps cells alive by suppressing p53/TP53 under normal conditions, but stimulates apoptosis by repressing CDKN1A under stress conditions. Acts as a negative regulator of telomerase. Has negative and positive effects on RNA polymerase II-dependent transcription. Function: E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. These substrates include FILIP1, p53/TP53, CDKN1A and TERT. Keeps cells alive by suppressing p53/TP53 under normal conditions, but stimulates apoptosis by repressing CDKN1A under stress conditions. Acts as a negative regulator of telomerase. Has negative and positive effects on RNA polymerase II-dependent transcription. Subunit: Interacts with p53/TP53 and CDKN1A. Interacts with TERT, modulating telomere length homeostasis. Tissue Specificity: Ubiquitous. Post-translational modifications: Auto-ubiquitinated; which leads to proteasomal degradation. Similarity: Contains 4 C3H1-type zinc fingers. Contains 1 RING-type zinc finger. SWISS: Q9UHC7 Gene ID: 23608 Database links: Entrez Gene: 23608 Human Entrez Gene: 54484 Mouse Entrez Gene: 296988 Rat Omim: 607754 Human SwissProt: Q9UHC7 Human SwissProt: Q9QXP6 Mouse Unigene: 728819 Human Unigene: 270484 Mouse
Product Picture	Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RNF61) Polyclonal Antibody, Unconjugated (SL9378R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RNF61) Polyclonal Antibody, Unconjugated (SL9378R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

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