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Rabbit Anti-PSMA5 antibody
Rabbit Anti-PSMA5 antibody
Macropain zeta chain; Multicatalytic endopeptidase complex zeta chain; Proteasome (prosome macropain) subunit alpha type 5; Proteasome alpha5 subunit; Proteasome component 5; Proteasome subunit alpha type 5; Proteasome subunit alpha type-5; Proteasome sub
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  • NO.:SL9354R
    Clonality:Polyclonal
    Immunogen Species:Rabbit
    React Species:Mouse,Rat,(predicted: Human,Chicken,Pig,Cow,Rabbit,)
    Applications:WB ELISA IHC-P IHC-F IF
    concentration:1mg/ml
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Product Name PSMA5
Chinese Name 蛋白酶体PSMα5抗体
Alias Macropain zeta chain; Multicatalytic endopeptidase complex zeta chain; Proteasome (prosome macropain) subunit alpha type 5; Proteasome alpha5 subunit; Proteasome component 5; Proteasome subunit alpha type 5; Proteasome subunit alpha type-5; Proteasome subunit zeta; Proteasome zeta chain; PSA5_HUMAN; PSC5; ZETA; Proteasome 20S alpha 5.  
Research Area Cell biology  Cyclin  Cell differentiation  
Immunogen Species Rabbit
Clonality Polyclonal
React Species Mouse, Rat,  (predicted: Human, Chicken, Pig, Cow, Rabbit, )
Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight 26.5kDa
Cellular localization The nucleus cytoplasmic 
Form Liquid
Concentration 1mg/ml
immunogen KLH conjugated synthetic peptide derived from human PSMA5: 31-130/241 
Lsotype IgG
Purification affinity purified by Protein A
Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
Product Detail The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.

Function:
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.

Subunit:
The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel. PSMA5 interacts directly with the PSMG1-PSMG2 heterodimer which promotes 20S proteasome assembly.

Subcellular Location:
Cytoplasm. Nucleus.

Tissue Specificity:
Expressed in fetal brain (at protein level).

Similarity:
Belongs to the peptidase T1A family.

SWISS:
P28066

Gene ID:
5686

Database links:

Entrez Gene: 5686 Human

Entrez Gene: 26442 Mouse

Entrez Gene: 29672 Rat

Omim: 176844 Human

SwissProt: P28066 Human

SwissProt: Q9Z2U1 Mouse

SwissProt: P34064 Rat

Unigene: 485246 Human

Unigene: 208883 Mouse

Unigene: 1276 Rat



Product Picture
Sample:
Lane 1: Thymus (Mouse) Tissue Lysate at 40 ug
Lane 2: NIH/3T3 (Mouse) Cell Lysate at 30 ug
Lane 3: Liver (Rat) Tissue Lysate at 40 ug
Lane 4: Thymus (Rat) Tissue Lysate at 40 ug
Primary:
Anti-PSMA5 (SL9354R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 26.5 kD
Observed band size: 27 kD

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