Product Name	SPO11
Chinese Name	减数分裂重组蛋白SPO11抗体
Alias	Meiotic recombination protein SPO 11; Meiotic recombination protein SPO11; MGC39953; SPO 11; SPO11 meiotic protein covalently bound to DSB homolog; SPO11 meiotic protein covalently bound to DSB like; SPO11_HUMAN.
Research Area	Tumour  Cell biology  Cyclin  Cell differentiation  Epigenetics
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Dog, Pig, Horse, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	45kDa
Cellular localization	The nucleus
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human SPO11: 151-330/396
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Spo11 is a type II topoisomerase that is thought to generate the chromosome breaks that initiate meiotic recombination. The Spo11 protein initiates meiotic recombination by generating DNA double-strand breaks (DSBs) and is required for meiotic synapsis in S. cerevisiae. The DSBs are located mostly in promoter regions, where the chromatin is in an open configuration, and cluster in domains along the chromosome. Expression of the Spo11 is detected mainly in the testis, in agreement with its predicted function in the initiation of meiotic recombination. Disruption of Spo11 leads to severe gonadal abnormalities from defective meiosis and results in infertility. Function: Required for meiotic recombination. Mediates DNA cleavage that forms the double-strand breaks (DSB) that initiate meiotic recombination (By similarity). Essential for the phosphorylation of SMC3, HORMAD1 and HORMAD2 (By similarity). Subcellular Location: Nuclear Tissue Specificity: Highly expressed in testis. Similarity: Belongs to the TOP6A family. SWISS: Q9Y5K1 Gene ID: 23626 Database links: Entrez Gene: 23626 Human Entrez Gene: 26972 Mouse Entrez Gene: 100125974 Pig Entrez Gene: 366261 Rat Omim: 605114 Human SwissProt: Q9Y5K1 Human SwissProt: Q9WTK8 Mouse Unigene: 159737 Human Unigene: 23495 Mouse
Product Picture	Sample: Lane 1: Mouse Testis tissue lysates Lane 2: Rat Testis tissue lysates Lane 3: Human PC-3 cell lysates Primary: Anti-SPO11 (SL9302R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 45 kDa Observed band size: 48 kDa

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