Product Name	RNF22/TRIM3
Chinese Name	Ring finger protein22抗体
Alias	TRIM3; BERP; Brain expressed RING finger protein; FLJ16135; HAC1; Ring finger protein 22; RNF22; RNF9; Tripartite motif containing 3
Research Area	Cell biology  immunology
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Rat,  (predicted: Human, Mouse, Dog, Pig, Cow, Horse, Sheep, )
Applications	ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	81kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human TRIM3/RNF22: 301-400/744
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	BERP; Brain expressed RING finger protein; FLJ16135; HAC1; Ring finger protein 22; RNF22; RNF9; Tripartite motif containing 3. Function: Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation. Subunit: Associates with myosin-Vb (MYO5B) and alpha-actinin-4 (ACTN4) (By similarity). Component of the CART complex, at least composed of ACTN4, HGS/HRS, MYO5B and TRIM3. Interacts with ZFYVE28/LST2. Subcellular Location: Cytoplasmic Tissue Specificity: Expressed in brain, heart, uterus and testis. Similarity: Belongs to the TRIM/RBCC family. Contains 1 B box-type zinc finger. Contains 1 filamin repeat. Contains 6 NHL repeats. Contains 1 RING-type zinc finger. SWISS: O75382 Gene ID: 10612 Database links: Entrez Gene: 10612 Human Entrez Gene: 55992 Mouse Entrez Gene: 83616 Rat Omim: 605493 Human SwissProt: O75382 Human SwissProt: Q9R1R2 Mouse SwissProt: O70277 Rat Unigene: 591992 Human Unigene: 29290 Mouse Unigene: 203982 Rat
Product Picture	Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RNF22/TRIM3) Polyclonal Antibody, Unconjugated (SL9174R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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