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Product Name TRIM7 Chinese Name 糖原生成素相互作用蛋白抗体 Alias Glycogenin-interacting protein; GNIP; RING finger protein 90; RNF90; TRIM7; TRIM7_HUMAN; Tripartite Motif Containing 7; Tripartite motif protein TRIM 7; Tripartite motif-containing protein 7. literatures Specific References (1) | SL9164R has been referenced in 1 publications.[IF=16.174] Liang, Xiao. et al. A C-terminal glutamine recognition mechanism revealed by E3 ligase TRIM7 structures. NAT CHEM BIOL. 2022 Aug;:1-10 WB, CoIP ; Human.Research Area Cell biology immunology Signal transduction Immunogen Species Rabbit Clonality Polyclonal React Species Human, (predicted: Mouse, Rat, Cow, Horse, Sheep, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 57kDa Cellular localization The nucleus cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human TRIM7/RNF90: 255-350/511 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail The tripartite motif (TRIM) family of proteins are characterized by a conserved TRIM domain that includes a coiled-coil region, a B-box type zinc finger, one RING finger and three zinc-binding domains. TRIM7 (tripartite motif-containing 7), also known as RNF90 or GNIP, is a 511 amino acid protein that belongs to the TRIM family and contains one RING-type zinc finger, one B box-type zinc finger and one SPRY domain. Expressed in placenta and skeletal muscle and present at lower levels in brain, heart and pancreas, TRIM7 localizes to both the cytoplasm and the nucleus where it exists as dimers and is thought to participate in the initiation of glycogen synthesis. Multiple isoforms of TRIM7 exist due to alternative splicing events.
Subunit:
Forms homodimers and heterodimers. Interacts with GYG and DES.
Tissue Specificity:
Skeletal muscle and placenta, at lower levels in heart, brain and pancreas. Isoform 1 is widely expressed with high level in testis, kidney and heart.
Similarity:
Belongs to the TRIM/RBCC family.
Contains 1 B box-type zinc finger.
Contains 1 B30.2/SPRY domain.
Contains 1 RING-type zinc finger.
SWISS:
Q9C029
Gene ID:
81786
Database links:Entrez Gene: 81786 Human
Omim: 609315 Human
SwissProt: Q9C029 Human
Unigene: 487412 Human
Product Picture Sample: Siha Cell (Human) Lysate at 40 ug
Primary: Anti-TRIM7 (SL9164R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 57 kD
Observed band size: 57 kD
Paraformaldehyde-fixed, paraffin embedded (Human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TRIM7) Polyclonal Antibody, Unconjugated (SL9164R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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