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Rabbit Anti-AHNAK2 antibody
Rabbit Anti-AHNAK2 antibody
AHNAK nucleoprotein 2; C14orf78; AHNAK 2; chromosome 14 open reading frame 78; KIAA2019; AHNK2_HUMAN.
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  • NO.:SL9094R
    Clonality:Polyclonal
    Immunogen Species:Rabbit
    React Species:Mouse,Rat,(predicted: Human,Dog,Cow,Horse,Rabbit,Sheep,)
    Applications:WB ELISA IHC-P IHC-F IF
    concentration:1mg/ml
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Details

Product Name AHNAK2
Chinese Name AHNAK核蛋白2抗体
Alias AHNAK nucleoprotein 2; C14orf78; AHNAK 2; chromosome 14 open reading frame 78; KIAA2019; AHNK2_HUMAN.  
Research Area Cell biology  immunology  
Immunogen Species Rabbit
Clonality Polyclonal
React Species Mouse, Rat,  (predicted: Human, Dog, Cow, Horse, Rabbit, Sheep, )
Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight 616kDa
Cellular localization The nucleus 
Form Liquid
Concentration 1mg/ml
immunogen KLH conjugated synthetic peptide derived from human AHNAK2: 1201-1300/5795 
Lsotype IgG
Purification affinity purified by Protein A
Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
Product Detail AHNAK2 contains 1 PDZ (DHR) domain. There are 3 named isoforms of AHNAK2 produced by alternative splicing. AHNAK2 is a 600-kDa protein composed of a large number of highly conserved repeat segments. AHNAKs are a class of giant propeller-like proteins that associate with calcium channel proteins of cardiomyocytes and other cells.

Subunit:
Interacts with DYSF; the interaction is direct and Ca(2+)-independent.

Subcellular Location:
Nuclear

Similarity:
Contains 1 PDZ (DHR) domain.

SWISS:
Q8IVF2

Gene ID:
113146

Database links:

Entrez Gene: 113146 Human

GenBank: BC049216 Human

Omim: 608570 Human

SwissProt: Q8IVF2 Human



Product Picture
Sample:
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti- AHNAK2 (SL9094R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 616 kD
Observed band size: 70 kD
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (AHNAK2) Polyclonal Antibody, Unconjugated (SL9094R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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