Product Name	hnRNP A2B1
Chinese Name	异质核糖核蛋白hnRNPA2抗体
Alias	Heterogeneous nuclear ribonucleoprotein A2; Heterogeneous nuclear ribonucleoprotein A2; Heterogeneous nuclear ribonucleoprotein A2/B1; Heterogeneous nuclear ribonucleoprotein A2/B1; Heterogeneous nuclear ribonucleoprotein B1; Heterogeneous nuclear ribonucleoprotein B1; Heterogeneous nuclear ribonucleoproteins A2/B1; hnRNP A2; hnRNP A2/hnRNP B1; hnRNP A2/B1; hnRNP B1; hnRNP-A2; hnRNP-B1; hnRNPA2; Hnrnpa2b1; hnRNPB1; HNRPA2B1; HNRPB1; Nuclear ribonucleoprotein particle A2 protein; Nuclear ribonucleoprotein particle A2 protein; RNP A2; RNP B1; RNP-A2; RNP-B1; RNPA2; RNPB1; ROA2_HUMAN; SNRPB1.
Research Area	Cell biology  immunology  Epigenetics
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse,  (predicted: Rat, Chicken, Dog, Pig, Cow, Rabbit, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	39kDa
Cellular localization	The nucleus cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human hnRNP A2B1: 101-200/353
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Involved with pre-mRNA processing. Forms complexes (ribonucleosomes) with at least 20 other different hnRNP and heterogeneous nuclear RNA in the nucleous. Function: Involved with pre-mRNA processing. Forms complexes (ribonucleosomes) with at least 20 other different hnRNP and heterogeneous nuclear RNA in the nucleus. Subunit: Identified in the spliceosome C complex. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Interacts with IGF2BP1. Subcellular Location: Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Component of ribonucleosomes. Predominantly nucleoplasmic, however isoform A2 is also found in the cytoplasm of cells in some tissues Similarity: Contains 2 RRM (RNA recognition motif) domains. SWISS: P22626 Gene ID: 3181 Database links: Entrez Gene: 3181 Human Entrez Gene: 100045191 Mouse Entrez Gene: 53379 Mouse Entrez Gene: 362361 Rat Omim: 600124 Human SwissProt: P22626 Human SwissProt: O88569 Mouse SwissProt: A7VJC2 Rat Unigene: 487774 Human Unigene: 155896 Mouse Unigene: 4057 Rat
Product Picture	Sample: A549(Human) Cell Lysate at 30 ug Primary: Anti-hnRNP A2B1 (SL9091R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 39 kD Observed band size: 39 kD Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (hnRNP A2B1) Polyclonal Antibody, Unconjugated (SL9091R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse colon tissue ); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (hnRNP A2B1) Polyclonal Antibody, Unconjugated (SL9091R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat intestine tissue ); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (hnRNP A2B1) Polyclonal Antibody, Unconjugated (SL9091R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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