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Product Name PAPSS1 Chinese Name 腺苷酸硫酸激酶1抗体 Alias Adenylyl-sulfate kinase; Adenylylsulfate 3''-phosphotransferase; APS kinase; ATP-sulfurylase; ATPSK 1; ATPSK1; Bifunctional 3' phosphoadenosine 5' phosphosulfate synthetase 1; PAPS synthase 1; PAPS synthetase 1; PAPS1_HUMAN; PAPSS 1; PAPSS; PAPSS1; SAT; SK 1; SK1; Sulfate adenylate transferase; Sulfurylase kinase 1; 3 prime phosphoadenosine 5 prime phosphosulfate synthase 1; 3' phosphoadenosine 5' phosphosulfate synthase 1; 3''-phosphoadenosine-5''-phosphosulfate synthase; Adenosine-5''-phosphosulfate 3''-phosphotransferase. Research Area Cell biology immunology Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, (predicted: Human, Rat, Dog, Cow, Horse, Rabbit, ) Applications ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 69kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human PAPSS1: 155-260/624 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Three-prime-phosphoadenosine 5-prime-phosphosulfate (PAPS) is the sulfate donor cosubstrate for all sulfotransferase (SULT) enzymes (Xu et al., 2000 [PubMed 10679223]). SULTs catalyze the sulfate conjugation of many endogenous and exogenous compounds, including drugs and other xenobiotics. In humans, PAPS is synthesized from adenosine 5-prime triphosphate (ATP) and inorganic sulfate by 2 isoforms, PAPSS1 and PAPSS2 (MIM 603005).[supplied by OMIM, Mar 2008].
Function:
Bifunctional enzyme with both ATP sulfurylase and APS kinase activity, which mediates two steps in the sulfate activation pathway. The first step is the transfer of a sulfate group to ATP to yield adenosine 5'-phosphosulfate (APS), and the second step is the transfer of a phosphate group from ATP to APS yielding 3'-phosphoadenylylsulfate (PAPS: activated sulfate donor used by sulfotransferase). In mammals, PAPS is the sole source of sulfate; APS appears to be only an intermediate in the sulfate-activation pathway. Also involved in the biosynthesis of sulfated L-selectin ligands in endothelial cells.
Tissue Specificity:
Expressed in testis, pancreas, kidney, thymus, prostate, ovary, small intestine, colon, leukocytes and liver. Also expressed in high endothelial venules (HEV) cells and in cartilage.
Similarity:
In the N-terminal section; belongs to the APS kinase family.
In the C-terminal section; belongs to the sulfate adenylyltransferase family.
SWISS:
O43252
Gene ID:
9060
Database links:Entrez Gene: 9060 Human
Entrez Gene: 9061 Human
Entrez Gene: 23971 Mouse
Omim: 603262 Human
SwissProt: O43252 Human
SwissProt: O95340 Human
SwissProt: O88428 Mouse
SwissProt: Q60967 Mouse
Unigene: 368610 Human
Unigene: 524491 Human
Unigene: 203916 Mouse
Unigene: 244912 Mouse
Unigene: 3507 Rat
Product Picture Paraformaldehyde-fixed, paraffin embedded (mouse pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PAPSS1) Polyclonal Antibody, Unconjugated (SL9029R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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