Product Name	Annexin VI
Chinese Name	膜粘连蛋白6抗体
Alias	Annexin VI; 67 kDa calelectrin; Annexin A6; Annexin VI p68; AnnexinA6; AnnexinVI; ANX 6; ANX A6; ANX6; ANXA 6; ANXA6; Calcium binding protein p68; Calelectrin; Calphobindin II; CalphobindinII; CBP 68; CBP68; Chromobindin 20; Chromobindin20; CPB II; CPBII; Lipocortin VI; LipocortinVI; p68; P70; Protein III; ProteinIII.
Research Area	Tumour  Cell biology  Binding protein  The cell membrane蛋白
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Mouse, Rat,  (predicted: Human, Chicken, Dog, Pig, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	67-74kDa
Cellular localization	The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human Annexin VI: 601-673/673
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The Annexin family of calcium binding proteins is composed of at least ten mammalian genes. It is characterised by a conserved core domain which binds to phospholipids in a Ca2+ dependent manner and a unique amino terminal region which may confer binding specificity. The Annexin family has been implicated as regulators of such diverse processes as ion flux, endocytosis and exocytosis, and cellular adhesion. When overexpressed in A431 cells, Annexin VI causes a partial reversal of the transformed phenotype. Function: May associate with CD21. May regulate the release of Ca(2+) from intracellular stores. Tissue Specificity: Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Post-translational modifications: Phosphorylated in response to growth factor stimulation. Similarity: Belongs to the annexin family. Contains 8 annexin repeats. SWISS: P08133 Gene ID: 309 Database links: Entrez Gene: 309 Human Entrez Gene: 11749 Mouse Entrez Gene: 79125 Rat Omim: 114070 Human SwissProt: P08133 Human SwissProt: P14824 Mouse SwissProt: P48037 Rat Unigene: 412117 Human Unigene: 265347 Mouse Unigene: 204053 Rat
Product Picture	Protein: uterus(mouse) lyates at 40ug; Primary: Rabbit Anti- Annexin VI (SL8997R) at 1:300; Secondary: 800CW Conjugated Goat (polyclonal) Anti-Rabbit IgG(H+L) at 1: 10000; Predicted band size:67/74 kD Observed band size:67 kD Sample: Heart (Mouse) Lysate at 40 ug Primary: Anti-Annexin VI (SL8997R )at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 67/74 kD Observed band size: 70 kD Paraformaldehyde-fixed, paraffin embedded (rat uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Annexin VI) Polyclonal Antibody, Unconjugated (SL8997R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

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