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Rabbit Anti-FLIP antibody
Rabbit Anti-FLIP antibody
c FLIP; c FLIPL; c FLIPR; c FLIPS; c-FLIP; c-FLIPL; c-FLIPR; c-FLIPS; CASH; CASP8 and FADD like apoptosis regulator; CASP8 and FADD-like apoptosis regulator subunit p12; CASP8AP1; Caspase homolog; Caspase like apoptosis regulatory protein; Caspase related
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  • NO.:SL8855R
    Clonality:Polyclonal
    Immunogen Species:Rabbit
    React Species:Human,Mouse,Rat,(predicted: Pig,Horse,Rabbit,)
    Applications:WB ELISA IHC-P IHC-F ICC IF
    concentration:1mg/ml
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Details

Product Name FLIP
Chinese Name 蛋白酶样凋亡调节蛋白CASH抗体
Alias c FLIP; c FLIPL; c FLIPR; c FLIPS; c-FLIP; c-FLIPL; c-FLIPR; c-FLIPS; CASH; CASP8 and FADD like apoptosis regulator; CASP8 and FADD-like apoptosis regulator subunit p12; CASP8AP1; Caspase homolog; Caspase like apoptosis regulatory protein; Caspase related inducer of apoptosis; Caspase-eight-related protein; Caspase-like apoptosis regulatory protein; Casper; Cellular FLICE-like inhibitory protein; CFLA; Cflar; CFLAR_HUMAN; CLARP; FADD like anti apoptotic molecule; FADD-like antiapoptotic molecule 1; FLAME; FLAME-1; FLAME1; I FLICE; I-FLICE; Inhibitor of FLICE; MACH-related inducer of toxicity; MRIT; Usurpin; Usurpin beta.  
Research Area Cell biology  Signal transduction  Apoptosis  
Immunogen Species Rabbit
Clonality Polyclonal
React Species Human, Mouse, Rat,  (predicted: Pig, Horse, Rabbit, )
Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight 43kDa
Cellular localization cytoplasmic 
Form Liquid
Concentration 1mg/ml
immunogen KLH conjugated synthetic peptide derived from human CFLIP: 1-100/480 
Lsotype IgG
Purification affinity purified by Protein A
Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
Product Detail The protein encoded by this gene is a regulator of apoptosis and is structurally similar to caspase-8. However, the encoded protein lacks caspase activity and appears to be itself cleaved into two peptides by caspase-8. Several transcript variants encoding different isoforms have been found for this gene, and partial evidence for several more variants exists. [provided by RefSeq, Feb 2011]

Function:
Apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells. Acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. Lacks enzymatic (caspase) activity.

Tissue Specificity:
Widely expressed. Higher expression in skeletal muscle, pancreas, heart, kidney, placenta, and peripheral blood leukocytes. Also detected in diverse cell lines. Isoform 8 is predominantly expressed in testis and skeletal muscle.

Post-translational modifications:
Proteolytically processed; probably by caspase-8. Processing likely occurs at the DISC and generates subunit p43 and p12.

Similarity:
Belongs to the peptidase C14A family.
Contains 2 DED (death effector) domains.

SWISS:
O15519

Gene ID:
8837

Database links:

Entrez Gene: 8837 Human

Entrez Gene: 12633 Mouse

Entrez Gene: 117279 Rat

Omim: 603599 Human

SwissProt: O15519 Human

SwissProt: O35732 Mouse

Unigene: 390736 Human

Unigene: 336848 Mouse



Product Picture
Sample:
Skeletal muscle(Mouse) Cell Lysate at 40 ug
Primary: Anti-CFLIP (SL8855R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 43 kD
Observed band size: 43 kD
Sample:
Muscle(Mouse) Cell Lysate at 40 ug
Primary: Anti-CFLIP (SL8855R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 43 kD
Observed band size: 43 kD
Sample:
Lane 1: Human Panc-1 cell lysates
Lane 2: Human Huvec cell lysates
Lane 3: Human K562 cell lysates
Lane 4: Human HepG2 cell lysates
Primary: Anti-FLIP (SL8855R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 43 kDa
Observed band size: 58 kDa
Paraformaldehyde-fixed, paraffin embedded (rat testis tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CFLAR) Polyclonal Antibody, Unconjugated (SL8855R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat heart tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CFLAR) Polyclonal Antibody, Unconjugated (SL8855R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CFLAR) Polyclonal Antibody, Unconjugated (SL8855R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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