Product Name	phospho-Smad2/Smad3 (Thr8)
Chinese Name	磷酸化细胞Signal transduction分子SMAD2/SMAD3抗体
Alias	Smad2 + Smad3 (phospho T8); p-Smad2 + Smad3 (phospho T8); P-Smad2 /3 (phospho T8); hMAD 2; hMAD 3; hSMAD2; hSMAD3; Mad related protein 2; MADH2; MADH3; MADR2; Mothers against DPP homolog 2; Mothers against DPP homolog 3; Sma and Mad related protein 2; SMA and MAD related protein 3; SMAD 2; SMAD 3; SMAD family member 2; SMAD family member 3.
literatures	Specific References  (8)     |     SL8853R has been referenced in 8 publications. [IF=5.682] Xiaolan You. et al. Fibroblastic galectin-1-fostered invasion and metastasis are mediated by TGF-β1-induced epithelial-mesenchymal transition in gastric cancer. Aging-Us. 2021 Jul 31; 13(14): 18464–18481  WB,IHC ;  Mouse.   PubMed:34260413 [IF=5.097] Yan L et al. Exosomes produced from 3D cultures of umbilical cord mesenchymal stem cells in a hollow-fiber bioreactor show improved osteochondral regeneration activity. Cell Biology and Toxicology.  WB ;  Human.   PubMed:doi:10.1007/s10565-019-09504-5 [IF=5.068] Chen XY et al. Pulsed Magnetic Field Stimuli Can Promote Chondrogenic Differentiation of Superparamagnetic Iron Oxide Nanoparticles-Labeled Mesenchymal Stem Cells in Rats.(2018) J Biomed Nanotechnol. 14(12):2135-2145.  WB ;  Rat.   PubMed:30305220 [IF=4.96] Liang H et al. RGFP966, a Histone deacetylase 3 inhibitor, promotes glioma stem cell differentiation by blocking TGF-β signaling via SMAD7. Biochem Pharmacol . 2020 Oct;180:114118.  WB ;  Human.   PubMed:32585142 [IF=4.423] Terzuoli E et al. Targeting endothelial-to-mesenchymal transition: the protective role of hydroxytyrosol sulfate metabolite. Eur J Nutr. 2019 Feb 6.  WB ;  Human.   PubMed:30725211 [IF=3.457] Gao H et al.Salvanic acid B inhibits myocardial fibrosis through regulating TGF-β1/Smad signaling pathway.(2018) Biomed. Pharmacother 110  IHC-P&WB ;  Mouse.   PubMed:30553195 [IF=2.826] Liang Yang. et al. TGFβ3 regulates adipogenesis of bovine subcutaneous preadipocytes via typical Smad and atypical MAPK signaling pathways. ELECTRON J BIOTECHN. 2023 Jan;61:33  WB ;  Bovine.   PubMed:10.1016/j.ejbt.2022.11.001 [IF=2.474] Yijun Liu. et al. The development of nasal polyps involves early middle meatus mucous remodeling via TGF-β1 mediated PAI-1 reduction. BRAZ J OTORHINOLAR. 2023 Feb;:  WB ;  Human.   PubMed:36841712
Product Type	Phosphorylated anti
Research Area	Tumour  Cell biology  immunology  Signal transduction  Apoptosis  transcriptional regulatory factor  Epigenetics
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse,  (predicted: Rat, Chicken, Dog, Pig, Cow, Horse, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test ICC=1:100 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	52kDa
Cellular localization	The nucleus cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthesised phosphopeptide derived from human Smad2/Smad3 around the phosphorylation site of Thr8: PF(p-T)PP
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, May 2012] Function: SMAD is a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. They mediate the signal of the transforming growth factor (TGF)-beta, and thus regulate multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. Subcellular Location: Cytoplasm. Nucleus. Note: Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4. SWISS: P84022 Gene ID: 4087 Database links: Entrez Gene: 4087 Human Entrez Gene: 4088 Human Entrez Gene: 17126 Mouse Entrez Gene: 17127 Mouse Entrez Gene: 25631 Rat Entrez Gene: 29357 Rat Omim: 601366 Human Omim: 603109 Human SwissProt: P84022 Human SwissProt: Q15796 Human SwissProt: Q62432 Mouse SwissProt: Q8BUN5 Mouse SwissProt: O70436 Rat SwissProt: P84025 Rat Unigene: 12253 Human Unigene: 714621 Human Unigene: 10636 Rat
Product Picture	Sample: U251 cell(human) Lysate at 30 ug Primary: Anti- p-Smad2/Smad3 (Thr8) (SL8853R) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52kD Observed band size: 52 kD Sample: cerebrum(mouse) Lysate at 40 ug RSC96 cell(rat) Lysate at 30 ug hepG2 cell(human) Lysate at 30 ug Hcclm3 cell(human) Lysate at 30 ug Primary: Anti- p-Smad2/Smad3 (Thr8) (SL8853R) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52kD Observed band size: 48,52 kD Sample: HUVEC cell(human) Lysate at 30 ug Primary: Anti- p-Smad2/Smad3 (Thr8) (SL8853R) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52kD Observed band size: 52 kD blank: A549 cells (blue line) isotype control: rabbit IgG (orange line) second antibody: goat anti-rabbit IgG (white blue line) primary antibody: rabbit Anti-phospho-Smad2/Smad3 (Thr8) (green line); contration: 3μg /10^6 cells Blank control: Hela. Primary Antibody (green line): Rabbit Anti-phospho-Smad2/Smad3 (Thr8) antibody (SL8853R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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