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Product Name HAVCR2/TIM-3 Chinese Name Tlymphocyte膜蛋白3(CD366)抗体 Alias Hepatitis A virus cellular receptor 2 homolog; CD366; CD 366; TIM-3; HAVR2_MOUSE; Kidney injury molecule 3; KIM 3; KIM3; T cell immunoglobulin and mucin domain containing 3; T cell immunoglobulin mucin 3; T-cell immunoglobulin and mucin domain-containing protein 3; T-cell immunoglobulin mucin receptor 3; T-cell membrane protein 3; TIM3; TIMD-3; TIM 3; TIMD3. literatures Specific References (3) | SL8766R has been referenced in 3 publications.[IF=4.068] Li Wang. et al. Radiotherapy upregulated immune checkpoints contribute to the development of second primary OSCC. ORAL DIS. 2023 May;: WB ; Rat.[IF=3.743] Zhu G et al. ShenQi FuZheng Injection ameliorates fatigue-like behavior in mouse models of cancer-related fatigue. Biomed Pharmacother. 2019 Mar;111:1376-1382. IHC ; Mouse.[IF=3.542] Guo Y et al. Tim-3 exacerbates kidney ischaemia/reperfusion injury through the TLR-4/NF-κB signalling pathway and an NLR-C4 inflammasome activation. Clinical & Experimental Immunology,2018 193(1), 113–129. IF ; Mouse.Research Area Cell biology immunology lymphocyte t-lymphocyte The cell membrane蛋白 Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, Rat, Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 29kDa Cellular localization The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from mouse HAVCR2: 151-250/281 <Extracellular> Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail The protein encoded by this gene belongs to the immunoglobulin superfamily, and TIM family of proteins. CD4-positive T helper lymphocytes can be divided into types 1 (Th1) and 2 (Th2) on the basis of their cytokine secretion patterns. Th1 cells are involved in cell-mediated immunity to intracellular pathogens and delayed-type hypersensitivity reactions, whereas, Th2 cells are involved in the control of extracellular helminthic infections and the promotion of atopic and allergic diseases. This protein is a Th1-specific cell surface protein that regulates macrophage activation, and inhibits Th1-mediated auto- and alloimmune responses, and promotes immunological tolerance. [provided by RefSeq, Sep 2011]
Function:
Regulates macrophage activation. Inhibits T-helper type 1 lymphocyte (Th1)-mediated auto- and alloimmune responses and promotes immunological tolerance. May be also involved in T-cell homing. Receptor for LGALS9.
Subcellular Location:
Membrane.
Tissue Specificity:
T-helper type 1 lymphocyte (Th1)-specific.
Post-translational modifications:
O-glycosylated with core 1 or possibly core 8 glycans.
Similarity:
Belongs to the immunoglobulin superfamily. TIM family.
SWISS:
Q8VIM0
Gene ID:
171285
Database links:Entrez Gene: 171285 Mouse
SwissProt: Q8VIM0 Mouse
Unigene: 72168 Mouse
Product Picture Sample:
Liver(Rat) Lysate at 40 ug
Primary: Anti-TIM 3 (SL8766R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 29 kD
Observed band size: 49 kD
Sample:
Thymus (Mouse) Lysate at 40 ug
Raw264.7(Mouse) Cell Lysate at 30 ug
Primary: Anti-TIM 3 (SL8766R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 29 kD
Observed band size: 49 kD
Sample:
Spleen (Mouse) Lysate at 40 ug
Primary: Anti-TIM 3 (SL8766R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 29 kD
Observed band size: 49 kD
Tissue/cell: rat liver tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-TIM-3 Polyclonal Antibody, Unconjugated(SL8766R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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