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Product Name Phospholipase D2 Chinese Name 胆碱磷酸酯酶2抗体 Alias Choline phosphatase 2; EC 3.1.4.4; hPLD 2; hPLD2; mPLD2; Phosphatidylcholine hydrolyzing phospholipase D2; Phosphatidylcholine-hydrolyzing phospholipase D2; Phospholipase D2; PhospholipaseD2; PLD 2; PLD1C; Pld2; PLD2_HUMAN; Pldc; rPLD2. literatures Specific References (1) | SL8747R has been referenced in 1 publications.[IF=3.647] Chang Y et al. The in vitro effects of phospholipase D1-mTOR axis in liver fibrogenesis. Life Sci . 2020 Jun 15;251:117595. IHC ; mouse.Research Area Tumour Cell biology Neurobiology Signal transduction Kinases and Phosphatases G protein signal Immunogen Species Rabbit Clonality Polyclonal React Species Human, (predicted: Mouse, Rat, Dog, Cow, Horse, Rabbit, Sheep, ) Applications ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 106kDa Cellular localization The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human PLD2/Phospholipase D2: 21-120/933 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Product Picture Paraformaldehyde-fixed, paraffin embedded (human pancreatic cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospholipase D2) Polyclonal Antibody, Unconjugated (SL8747R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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