Product Name	Epac1
Chinese Name	环磷酸腺苷调节鸟嘌呤核苷酸交换因子1抗体
Alias	bcm910; CAMP GEFI; cAMP regulated guanine nucleotide exchange factor I; CAMPGEFI; CGEF 1; CGEF1; EPA1; Epac 1; EPAC; EPAC1; Exchange factor directly activated by cAMP 1; Exchange protein directly activated by cAMP 1; MGC21410; RAP guanine nucleotide exchange factor; Rap guanine nucleotide exchange factor (GEF) 3; RAP guanine nucleotide exchange factor 3; Rap1 guanine nucleotide exchange factor directly activated by cAMP; RAPGEF3.
literatures	Specific References  (2)     |     SL8682R has been referenced in 2 publications. [IF=3.056] Fanfan Sun. et al. Epac1 signaling pathway mediates the damage and apoptosis of inner ear hair cells after noise exposure in a rat model. Neuroscience. 2021 Apr;:  IF ;  Rat.   PubMed:33838290 [IF=0] Zhang C et al. IFN-stimulated P2Y13 protects mice from viral infection by suppressing the cAMP/EPAC1 signaling pathway. Res Vet Sci. 2018 Oct;120:4-10.  WB ;  Human&Mouse.   PubMed:30137373
Research Area	immunology  Neurobiology  Signal transduction
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Rat,  (predicted: Mouse, Rabbit, )
Applications	ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	99kDa
Cellular localization	The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human Epac1: 301-400/923
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The activation of RaP1 by cAMP is independent of PKA and is mediated by recently discovered family of guanine nucleotide exchange factors (GEFs) called cAMP-GEFs or Epacs. The Epac signaling therefore represents a novel mechanism for cAMP signaling with in the cAMP cascade. There are 2 members of the Epac family, Epac1 and Epac 2. Both proteins are multidomain proteins containing an autoinhibitory cAMP-binding domain that inhibits the catalytic region and a DEP domain (dishevelled, Egl-10 and pleckstrin homology domain) targeting the membrane anchors. EPAC2 has an additional cAMP-binding site in its N-terminus that binds cAMP with low affinity. EPAC1 mRNA is broadly expressed, with particularly high levels occurring in the thyroid, ovary, kidney and certain brain regions, whereas expression of EPAC2 mRNA appears to be restricted to the brain and adrenal glands. Epac 1 and Epac 2 also interact with light chain 2 (LC2) or MAP1A that serves as a scaffolding structure to stabilize the signal transduction complex. The Epac 1-selective antibodies were generated against unique antigenic sequences form near N-terminus and between RasGEFN and Ras GEF domains. The antibodies to Epac 1are affinity purified over immobilized antigen based chromatography. Function: Guanine nucleotide exchange factor (GEF) for RAP1A and RAP2A small GTPases that is activated by binding cAMP. Through simultaneous binding of PDE3B to RAPGEF3 and PIK3R6 is assembled in a signaling complex in which it activates the PI3K gamma complex and which is involved in angiogenesis. Subunit: Interacts with PDE3B. Subcellular Location: Endomembrane system Tissue Specificity: Widely expressed with highest levels in adult kidney, heart, thyroid and brain, and fetal kidney. Similarity: Contains 1 cyclic nucleotide-binding domain. Contains 1 DEP domain. Contains 1 N-terminal Ras-GEF domain. Contains 1 Ras-GEF domain. SWISS: O95398 Gene ID: 10411 Database links: Entrez Gene: 10411 Human Entrez Gene: 223864 Mouse Entrez Gene: 59326 Rat Omim: 606057 Human SwissProt: O95398 Human SwissProt: Q8VCC8 Mouse SwissProt: Q9Z1C8 Rat Unigene: 8578 Human Unigene: 42899 Rat
Product Picture	Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Epac1) Polyclonal Antibody, Unconjugated (SL8682R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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