Product Name	Ceramide glucosyltransferase
Chinese Name	葡萄糖神经酰胺合成酶抗体
Alias	CEGT_HUMAN; UGCG; GCS; GLCT 1; GLCT-1; GLCT1; Glucosylceramide synthase; UDP glucose ceramide glucosyltransferase; UDP glucose N acylsphingosine D glucosyltransferase; UDP-glucose ceramide glucosyltransferase; UDP-glucose:N-acylsphingosine D-glucosyltransferase; Ugcg.
literatures	Specific References  (2)     |     SL8593R has been referenced in 2 publications. [IF=5.923] Pietro Barbacini. et al. Novel Insight into the Serum Sphingolipid Fingerprint Characterizing Longevity. Int J Mol Sci. 2022 Jan;23(5):2428  WB ;  Human.   PubMed:10.3390/ijms23052428 [IF=4.757] Tami Igarashi. et al. Horse-Derived Ceramide Accentuates Glucosylceramide Synthase and Ceramide Synthase 3 by Activating PPARβ/δ and/or PPARγ to Stimulate Ceramide Synthesis. BIOMEDICINES. 2023 Feb;11(2):548  WB ;  Human.   PubMed:36831084
Research Area	Tumour  Cell biology  Apoptosis  Cell differentiation
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Chicken, Dog, Pig, Cow, Horse, Rabbit, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	45kDa
Cellular localization	cytoplasmic The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human UGCG: 31-98/394
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Glucosylceramide synthase (GCS), also designated ceramide glucosyltransferase, belongs to the glycosyltransferase 2 family. It is a widely expressed integral membrane protein encoded by UGCG. The enzyme can be found in the plasma membrane of all eukaryotic cells, and a significant concentration of glucosylceramide synthase activity has been reported in the Golgi complex. Glucosylceramide synthase catalyzes the first glycosylation step in glycosphingolipid biosynthesis and functions as a glucosyltransferase and flippase in the transfer of glucose to ceramide. Glucosylceramide synthase operates in cell recognition, cell proliferation and differentiation, immune recognition and signal transduction. The regulation of ceramide levels through glucosylceramide synthase has been associated with the induction of apoptosis and notable research implicates this relationship with drug-induced apoptosis in a variety of cell types. Function: Catalyzes the first glycosylation step in glycosphingolipid biosynthesis, the transfer of glucose to ceramide. May also serve as a "flippase". Subcellular Location: Golgi apparatus membrane. Tissue Specificity: Found in all tissues examined. Similarity: Belongs to the glycosyltransferase 2 family. SWISS: Q16739 Gene ID: 7357 Database links: Entrez Gene: 7357 Human Entrez Gene: 22234 Mouse Entrez Gene: 83626 Rat Omim: 602874 Human SwissProt: Q16739 Human SwissProt: O88693 Mouse SwissProt: Q9R0E0 Rat Unigene: 304249 Human Unigene: 593014 Human Unigene: 198803 Mouse Unigene: 24091 Rat
Product Picture	Sample: Lymph node (Mouse) Lysate at 40 ug Primary: Anti-Ceramide glucosyltransferase (SL8593R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 45 kD Observed band size: 45 kD Sample: Small intestine (Mouse) Lysate at 40 ug Primary: Anti- Ceramide glucosyltransferase (SL8593R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 45 kD Observed band size: 45 kD Paraformaldehyde-fixed, paraffin embedded (rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Ceramide glucosyltransferase) Polyclonal Antibody, Unconjugated (SL8593R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat small intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Ceramide glucosyltransferase) Polyclonal Antibody, Unconjugated (SL8593R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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