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Product Name phospho-CstF64 (Ser498) Chinese Name 磷酸化细胞分裂相关蛋白CSTF64抗体 Alias p-CstF-64 (Ser498); p-CstF-64 (S498); CstF64 (phospho-Ser498); CstF64 (phospho-S498); CSTF2T; CF1 64 kDa subunit, tau variant; Cleavage stimulation factor 64 kDa subunit, tau variant; CSTF 64 kDa subunit, tau variant; KIAA0689; TauCstF64; CSTF2_HUMAN. Product Type Phosphorylated anti Research Area Tumour Cell biology Cell differentiation Epigenetics Immunogen Species Rabbit Clonality Polyclonal React Species Human, (predicted: Mouse, Rat, Dog, Pig, Cow, Horse, Sheep, ) Applications ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 61kDa Cellular localization The nucleus Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthesised phosphopeptide derived from human CstF64 around the phosphorylation site of Ser498: QG(p-S)RQ Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Polyadenylation of mRNA precursors is a two-step reaction that requires multiple protein factors. The first step, endonucleolytic cleavage of polyadenylation substrates, requires CstF (cleavage stimulation factor), a heterotrimer that is composed of three distinct subunits. CstF-64 contains an RNA binding domain and is responsible for the RNA binding activity of CstF. CstF-64 is expressed in all somatic cells and in pre- and postmeiotic, but not meiotic, germ cells. However, a large variant of CstF-64, called t CstF-64, is abundantly expressed in meiotic and postmeiotic cells in the testis and to a lesser extent in the brain, and promotes the germ cell pattern of polyadenylation. The gene encoding CstF-64 (designated CSTF2) maps to the X chromosome, whereas t CstF-64 is encoded by an autosomal gene. The increase in CstF-64 concentration during B cell activation switches IgM heavy chain mRNA expression from membrane-bound to secreted forms, suggesting that CstF-64 plays a key role in regulating IgM heavy chain expression during B cell differentiation.
Function:
One of the multiple factors required for polyadenylation and 3'-end cleavage of mammalian pre-mRNAs. This subunit is directly involved in the binding to pre-mRNAs (By similarity).
Subunit:
The CSTF complex is composed of CSTF1 (50 kDa subunit), CSTF2 (64 kDa subunit) and CSTF3 (77 kDa subunit). CSTF2 directly interacts with CSTF3, SYMPK and RPO2TC1. Interacts with HSF1 in heat-stressed cells. Interacts with CPSF2, CPSF3 and FIP1L1. Interacts with DDX1.
Subcellular Location:
Nuclear
Similarity:
Contains 1 RRM (RNA recognition motif) domain.
SWISS:
P33240
Gene ID:
1478
Database links:Entrez Gene: 1478 Human
Omim: 300907 Human
SwissProt: P33240 Human
Unigene: 132370 Human
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