Product Name	CLEC 4E
Chinese Name	C型凝集素4家族E抗体
Alias	C type lectin domain family 4 member E; C type lectin superfamily member 9; C-type (calcium dependent carbohydrate recognition domain) lectin superfamily member 9; C-type lectin domain family 4 member E; C-type lectin superfamily member 9; CLC4E_HUMAN; CLEC 4E; CLEC4E Clec4e; CLECSF9; Macrophage inducible C type lectin; Macrophage-inducible C-type lectin; MINCLE.
literatures	Specific References  (6)     |     SL8541R has been referenced in 6 publications. [IF=11.864] Darja Andreev. et al. Osteocyte necrosis triggers osteoclast-mediated bone loss through macrophage-inducible C-type lectin. J Clin Invest. 2020 Sep;130(9):4811-4830  IF ;  Mouse.   PubMed:32773408 [IF=5.72] He, Yue, et al. "Mincle/Syk Signaling Pathway Contributes to Neuroinflammation after Subarachnoid Hemorrhage in Rats." Stroke; a journal of cerebral circulation 46.8 (2015): 2277.  WB, IHC ;  Rat.   PubMed:26138128 [IF=4.302] Wang Y et al. Microglial Mincle receptor in the PVN contributes to sympathetic hyperactivity in acute myocardial infarction rat.(2018) J Cell Mol Med. Oct 24.  IF,WB ;  Rat.   PubMed:30353660 [IF=3.201] Yuki Sato. et al. Limited Role of Mincle in the Host Defense against Infection with Cryptococcus deneoformans. Infect Immun. 2020 Oct;88(11):e00400-20  IHC ;  Mouse.   PubMed:32868343 [IF=2.234] Liu XY et al. Acupuncture through (DU20) to (GB7) mitigates neurological impairment after intracerebral hemorrhage. Neural Regen Res. 2018 Aug;13(8):1425-1432.  IHC-P&WB ;  Rat.   PubMed:30106055 [IF=1.166] Yu GR et al. Mincle in the innate immune response of mice fungal keratitis.Int J Ophthalmol. 2018 Apr 18;11(4):539-547.  IF ;  Mouse.   PubMed:29675368
Research Area	Cell biology  Signal transduction  Apoptosis
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human,  (predicted: Mouse, Rat, Cow, Horse, )
Applications	ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=2μg/Test IF=1:50-200 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	25kDa
Cellular localization	The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human CLECSF9: 51-150/219 <Extracellular>
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	C-type lectin that functions as cell-surface receptor for a wide variety of ligands such as damaged cells, fungi and mycobacteria. Plays a role in the recognition of pathogenic fungi, such as Candida albicans. The detection of mycobacteria is via trehalose 6,6'-dimycolate (TDM), a cell wall glycolipid. Specifically recognizes alpha-mannose residues on pathogenic fungi of the genus Malassezia. Recognizes also SAP130, a nuclear protein, that is released by dead or dying cells. Transduces signals through an ITAM-containing adapter protein, Fc receptor gamma chain /FCER1G. Induces secretion of inflammatory cytokines through a pathway that depends on SYK, CARD9 and NF-kappa-B. Function: C-type lectin that functions as cell-surface receptor for a wide variety of ligands such as damaged cells, fungi and mycobacteria. Plays a role in the recognition of pathogenic fungi, such as Candida albicans. The detection of mycobacteria is via trehalose 6,6'-dimycolate (TDM), a cell wall glycolipid. Specifically recognizes alpha-mannose residues on pathogenic fungi of the genus Malassezia. Recognizes also SAP130, a nuclear protein, that is released by dead or dying cells. Transduces signals through an ITAM-containing adapter protein, Fc receptor gamma chain /FCER1G. Induces secretion of inflammatory cytokines through a pathway that depends on SYK, CARD9 and NF-kappa-B. Subunit: Monomer. Homodimer. Subcellular Location: Membrane. Similarity: Contains 1 C-type lectin domain. SWISS: Q9ULY5 Gene ID: 26253 Database links: Entrez Gene: 26253 Human Omim: 609962 Human SwissProt: Q9ULY5 Human Unigene: 236516 Human
Product Picture	Blank control:THP-1. Primary Antibody (green line): Rabbit Anti-CLEC 4E antibody (SL8541R) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-FITC Dilution: 0.5μg /test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. Black line : Positive blank control (Hela); Negative blank control (A431） Green line : Primary Antibody (Rabbit Anti-CLEC 4E antibody (SL8541R) ) Orange line：Isotype Control Antibody (Rabbit IgG) . Blue line : Secondary Antibody (Goat anti-rabbit IgG-PE)/(Goat anti-rabbit IgG-AF488) Hela（Positive）and A431（Negative control）cells (black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CLEC 4E Antibody(SL8541R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange). Blank control: Hela(blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice. Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 5μg in 100 μL1X PBS containing 0.5% BSA(green).

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