Product Name	RNF210
Chinese Name	Ring finger protein210抗体
Alias	RNF210; Ret finger protein like 4; Ret finger protein-like 4A; RFPL4; RFPL4A; RFPLA_HUMAN; RING finger protein 210.
Research Area	Tumour  Cell biology  immunology
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Rat,  (predicted: Mouse, Pig, Horse, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	32kDa
Cellular localization	The nucleus cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human RFPL4A/RNF210: 101-200/287
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	RFPL4A is a 287 amino acid protein. RFPL4A contains a cysteine-rich RING finger-like region (C3YC4), a coiled-coil motif, and B30.2 domains characteristic of the RING-B30 family of E3 ubiquitin-protein ligases, such as MID1. The RFPL4A gene maps to human chromosome 19q13.42. Chromosome 19 consists of approximately 63 million bases and makes up over 2% of human genomic DNA. Chromosome 19 includes a diversity of interesting genes and is recognized for having the greatest gene density of the human chromosomes. It is the genetic home for a number of immunoglobulin superfamily members including the killer cell and leukocyte Ig-like receptors, a number of ICAMs, the CEACAM and PSG families, and Fc?receptors. Key genes for eye color and hair color also map to chromosome 19. Peutz-Jeghers syndrome, spinocerebellar ataxia type 6, the stroke disorder CADASIL, hypercholesterolemia and insulin-dependent diabetes have been linked to chromosome 19. Similarity: Contains 1 B30.2/SPRY domain. Contains 1 RING-type zinc finger. SWISS: A6NLU0 Gene ID: 342931 Database links: Entrez Gene: 342931 Human Omim: 612601 Human SwissProt: A6NLU0 Human Unigene: 631553 Human
Product Picture	Sample: MCF-7(Human) Cell Lysate at 30 ug Primary: Anti- RNF210 (SL8494R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 32 kD Observed band size: 33 kD Sample: MCF-7(Human) Cell Lysate at 30 ug Primary: Anti- RNF210 (SL8494R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 32 kD Observed band size: 32 kD Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RNF210) Polyclonal Antibody, Unconjugated (SL8494R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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