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Product Name FNDC8 Chinese Name Ⅲ型纤维连接蛋白域蛋白8抗体 Alias Fibronectin type III domain-containing protein 8; FNDC8; FNDC8_HUMAN; DKFZp434H2215. Research Area Cell biology immunology Immunogen Species Rabbit Clonality Polyclonal React Species Rat, (predicted: Human, Mouse, Dog, Pig, Cow, Rabbit, Sheep, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 36kDa Cellular localization The nucleus Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human FNDC8: 201-300/324 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail FNDC8 is a 324 amino acid protein containing one fibronectin type-III domain. Conserved in human, chimpanzee, dog, cow, mouse and rat, FNDC8 is encoded by a gene located on human chromosome 17q12. Human chromosome 17 comprises over 2.5% of the human genome and encodes over 1,200 genes. Two key tumor suppressor genes are associated with chromosome 17, namely, p53 and BRCA1.
Similarity:
Contains 1 fibronectin type-III domain.
SWISS:
Q8TC99
Gene ID:
54752
Database links:Entrez Gene: 54752 Human
SwissProt: Q8TC99 Human
Unigene: 120369 Human
Product Picture Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FNDC8) Polyclonal Antibody, Unconjugated (SL8488R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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