Product Name	phospho-GluR1 (Ser849)
Chinese Name	磷酸化谷氨酸受体1抗体
Alias	GluR1 (phospho Ser849); GluR1 (phospho S849); p- GluR1(Ser849); p- GluR1(S849); GLUR 1; GLUR A; AMPA 1; AMPA selective glutamate receptor 1; GLUH 1; GLUH1; GluR K1; GluR-1; GluR-A; GluR-K1; GLUR1; GLURA ; GluRK1; Glutamate receptor 1; Glutamate receptor ionotropic AMPA 1; Gria 1; Gria1 ; Gria1; HBGR1; MGC133252.
Product Type	Phosphorylated anti
Research Area	Tumour  Cell biology  immunology  Neurobiology  Signal transduction  Apoptosis  transcriptional regulatory factor
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Chicken, Dog, Pig, Cow, Horse, Sheep, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	98kDa
Cellular localization	cytoplasmic The cell membrane Extracellular matrix
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthesised phosphopeptide derived from human GluR1 around the phosphorylation site of Ser849: QQ(p-S)IN
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes with multiple subunits, each possessing transmembrane regions, and all arranged to form a ligand-gated ion channel. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. This gene belongs to a family of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) receptors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]. Function: Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate. Subunit: Homotetramer or heterotetramer of pore-forming glutamate receptor subunits. Tetramers may be formed by the dimerization of dimers. Interacts with DLG1 via its C-terminus. Interacts with SYNDIG1 and GRIA2. Interacts with LRFN. Interacts with HIP1 and RASGRF2. Found in a complex with GRIA2, GRIA3, GRIA4, CNIH2, CNIH3, CACNG2, CACNG3, CACNG4, CACNG5, CACNG7 and CACNG8. Interacts with CACNG5. Interacts with CNIH2 and CACNG2. Subcellular Location: Cell membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell projection, dendrit. Cell projection, dendritic spine. Note=Interaction with CACNG2, CNIH2 and CNIH3 promotes cell surface expression. Tissue Specificity: Widely expressed in brain. Post-translational modifications: Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-603 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-829 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis. Similarity: Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA1 subfamily. SWISS: P42261 Gene ID: 2890 Database links: Entrez Gene: 2890 Human Entrez Gene: 14799 Mouse Entrez Gene: 50592 Rat Omim: 138248 Human SwissProt: P42261 Human SwissProt: P23818 Mouse SwissProt: P19490 Rat Unigene: 519693 Human Unigene: 4920 Mouse Unigene: 29971 Rat NMDAR1又称GluR1 (Glutamate Receptor 1)近年实验研究发现，许多NMDAR拮抗药均具有镇痛活性，表明NMDAR在痛觉传递中具有重要作用，这为新型镇痛药的研究开发提供了新的作用靶点。
Product Picture	Sample: Brain (Mouse) Lysate at 40 ug Cerebellum (Mouse) Lysate at 40 ug Cerebellum (Rat) Lysate at 40 ug U251 (Human) Lysate at 30 ug Primary: Anti- phospho-GluR1 (Ser849) (SL8446R) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 98 kD Observed band size: 130 kD Sample: Cerebrum (Mouse) Lysate at 40 ug Cerebellum (Mouse) Lysate at 40 ug U251 (Human) Lysate at 30 ug Primary: Anti-phospho-GluR1 (Ser849) (SL8446R) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 98 kD Observed band size: 128 kD Sample: Cerebrum (Mouse) Lysate at 40 ug Cerebellum (Mouse) Lysate at 40 ug Primary: Anti-phospho-GluR1 (Ser849) (SL8446R) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 98 kD Observed band size: 128 kD Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-GluR1 (Ser849)) Polyclonal Antibody, Unconjugated (SL8446R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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