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Product Name SHFM3 Chinese Name SHFM3蛋白抗体 Alias DAC; Dactylin; F box and WD 40 domain containing protein 4; F box and WD 40 domain protein 4; F box and WD repeat domain containing 4; F box/WD repeat containing protein 4; F box/WD repeat protein 4; F-box and WD-40 domain-containing protein 4; F-box/WD repeat-containing protein 4; FBW 4; FBW4; FBWD 4; FBWD4; FBXW 4; FBXW4; FBXW4_HUMAN; SHFM 3; SHSF 3; SHSF3; Split hand/foot malformation (ectrodactyly) type. Research Area Cell biology Developmental biology Immunogen Species Rabbit Clonality Polyclonal React Species Rat, (predicted: Human, Mouse, ) Applications ELISA=1:5000-10000 IHC-P=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 46kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human SHFM3: 171-270/412 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Probably recognizes and binds to some phosphorylated proteins and promotes their ubiquitination and degradation. Likely to be involved in key signaling pathways crucial for normal limb development. May participate in Wnt signaling.
Involvement in disease:Defects in FBXW4 are a cause of split-hand/foot malformation type 3 (SHFM3) . SHFM3 is an autosomal dominant disorder characterized by hypoplasia/aplasia of the central digits with fusion of the remaining digits.
Function:
Probably recognizes and binds to some phosphorylated proteins and promotes their ubiquitination and degradation. Likely to be involved in key signaling pathways crucial for normal limb development. May participate in Wnt signaling.
Subunit:
Part of a SCF (SKP1-cullin-F-box) protein ligase complex (By similarity).
Subcellular Location:
Expressed in brain, kidney, lung and liver.
Tissue Specificity:
Expressed in brain, kidney, lung and liver.
DISEASE:
Defects in FBXW4 are a cause of split-hand/foot malformation type 3 (SHFM3) [MIM:246560]. SHFM3 is an autosomal dominant disorder characterized by hypoplasia/aplasia of the central digits with fusion of the remaining digits.
Similarity:
Contains 1 F-box domain.
Contains 4 WD repeats.
SWISS:
P57775
Gene ID:
6468
Database links:Entrez Gene: 6468 Human
Omim: 608071 Human
SwissProt: P57775 Human
Unigene: 500822 Human
Product Picture Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SHFM3) Polyclonal Antibody, Unconjugated (SL8390R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
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