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Product Name UBE2J2 Chinese Name Ubiquitin蛋白连接酶J2抗体 Alias NCUBE-2; NCUBE2; Non-canonical ubiquitin-conjugating enzyme 2; UB2J2_HUMAN; UBC6; UBC6 homolog; ube2j2; Ubiquitin-conjugating enzyme E2 J2. Research Area Cell biology immunology Signal transduction Epigenetics Immunogen Species Rabbit Clonality Polyclonal React Species Human, (predicted: Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 29kDa Cellular localization cytoplasmic The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human UBE2J2: 1-100/259 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Ubiquitination is an important molecular mechanism by which abnormal or short-lived proteins are targeted for degradation by the concerted efforts of at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). UBE2J2 (Ubiquitin-conjugating enzyme E2 J2), also known as NCUBE2 (Non-canonical ubiquitin-conjugating enzyme 2), is a 259 amino acid single pass type IV membrane protein that that belongs to the E2 ubiquitin-conjugating enzyme family and is involved in protein degradation. Localized to the membrane of the endoplasmic reticulum (ER), UBE2J2 catalyzes the attachment of ubiquitin to misfolded membrane proteins, thereby targeting them for proteasomal destruction. This ATP-dependent reaction yields AMP, a diphosphate and a ubiquitin-tagged protein and may be a method of quality control within the ER. Two isoforms of UBE2J2 exist due to alternative splicing events.
Function:
Catalyzes the covalent attachment of ubiquitin to other proteins. Seems to function in the selective degradation of misfolded membrane proteins from the endoplasmic reticulum (ERAD) (By similarity).
Subcellular Location:
Endoplasmic reticulum membrane; Single-pass type IV membrane protein (By similarity).
Similarity:
Belongs to the ubiquitin-conjugating enzyme family.
SWISS:
Q8N2K1
Gene ID:
118424
Database links:Entrez Gene: 118424 Human
Entrez Gene: 140499 Mouse
SwissProt: Q8N2K1 Human
SwissProt: Q6P073 Mouse
Unigene: 191987 Human
Unigene: 371673 Mouse
Product Picture Sample:
293T(Human) Cell Lysate at 40 ug
Primary: Anti-UBE2J2 (SL8381R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 29 kD
Observed band size: 29 kD
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