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Product Name NFX1 Chinese Name 核转录因子X盒Binding protein1抗体 Alias NFX2; MGC20369; NFX 1; NFX1; Nuclear transcription factor X box binding 1; Nuclear transcription factor X box binding protein 1; Transcriptional repressor NF X1; NFX1_HUMAN. Research Area Cell biology immunology Signal transduction transcriptional regulatory factor Epigenetics Immunogen Species Rabbit Clonality Polyclonal React Species Human, (predicted: Mouse, Rat, ) Applications WB=1:500-2000 ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 124kDa Cellular localization The nucleus Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human NFX1: 881-980/1120 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail NFX1 is a transcriptional repressor capable of binding to the conserved X box motif of HLA-DRA and other MHC class II genes in vitro. It may play a role in the inflammatory response, regulating its duration by limiting the period in which class II MHC molecules are induced by IFN gamma. The RING type zinc finger domain interacts with an ubiquitin conjugating enzyme (E2) and facilitates ubiquitination.
Function:
Binds to the X-box motif of MHC class II genes and represses their expression. May play an important role in regulating the duration of an inflammatory response by limiting the period in which MHC class II molecules are induced by interferon-gamma. Isoform 3 binds to the X-box motif of TERT promoter and represses its expression. Together with PABPC1 or PABPC4, isoform 1 acts as a coactivator for TERT expression. Mediates E2-dependent ubiquitination.
Subunit:
Isoform 1 and isoform 3 interact with human papillomavirus (HPV) type-16 E6 oncoprotein. Isoform 1 interacts with PABPC1 and PABPC4.
Subcellular Location:
Nucleus.
Post-translational modifications:
Isoform 3 is polyubiquitinated in the presence of HPV16 E6 protein; which leads to proteasomal degradation. Isoform 1 is not polyubiquitinated.
Similarity:
Belongs to the NFX1 family.
Contains 8 NF-X1-type zinc fingers.
Contains 1 R3H domain.
Contains 1 RING-type zinc finger.
SWISS:
Q12986
Gene ID:
4799
Database links:Entrez Gene: 4799 Human
Entrez Gene: 74164 Mouse
Omim: 603255 Human
SwissProt: Q12986 Human
SwissProt: B1AY10 Mouse
Unigene: 413074 Human
Unigene: 247456 Mouse
Product Picture Sample:
HL-60(Human) Cell Lysate at 30 ug
K562(Human) Cell Lysate at 30 ug
Primary: Anti-NFX1 (SL8377R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 124 kD
Observed band size: 124 kD
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