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Product Name Ube2G1 Chinese Name Ubiquitin蛋白连接酶G1抗体 Alias E217K; UB2G1_HUMAN; UBC 7; UBC7; UBC7 homolog yeast; UBE 2G; UBE2G; Ube2g1; Ubiquitin carrier protein G1; Ubiquitin conjugating enzyme E2 G1; Ubiquitin conjugating enzyme E2G 1 (homologous to C. elegans UBC7); Ubiquitin conjugating enzyme E2G 1 (UBC7 homolog C. elegans); Ubiquitin conjugating enzyme E2G 1 (UBC7 homolog yeast); Ubiquitin conjugating enzyme E2G 1; Ubiquitin protein ligase G1; Ubiquitin-conjugating enzyme E2 G1; Ubiquitin-protein ligase G1. literatures Specific References (1) | SL8371R has been referenced in 1 publications.[IF=4.586] Berentsen B et al. MicroRNA-34a Acutely Regulates Synaptic Efficacy in the Adult Dentate Gyrus In Vivo. Mol Neurobiol. 2019 Nov 21. WB ; Rat.Research Area Cell biology immunology Epigenetics Immunogen Species Rabbit Clonality Polyclonal React Species Rat, (predicted: Mouse, Dog, Pig, Horse, Rabbit, Sheep, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 19kDa Cellular localization cytoplasmic Extracellular matrix Secretory protein Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human Ube2G1/UBC7: 101-170/170 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Ubiquitin is an abundant, highly conserved protein found in all eukaryotic cells either free or covalently attached to cellular proteins. The primary function of ubiquitin in mammalian systems is to clear abnormal, foreign, and improperly folded proteins by targeting them for proteosome degradation. In Saccharomyces cerevisiae, ubiquitin-like proteins include Rub1, Ula1, Uba3, Smt3, Ubc2, Ubc12 and Ubc9. Rub1 shares 53% homology with ubiquitin and requires activation via the E2 proteins, including Ula1, Uba3 and Ubc12 in order to conjugate to substrates directed to different proteolytic systems. Ubc4 catalyzes ubiquitination of IkBa in a phosphorylation and SCFB-TRCP dependent manner. In this particular reaction, E1 first transfers ubiquitin to the E2 component Ubc4, and Ubc4 then associates with E3 ligase, which conjugates the poly-ubiquitin chain on a target protein. In this fashion, the chain tags the IkBa for degradation by a proteasome thus lifting the inhibitory effect of IkBa on NFkB and allowing NFkB to enter the nucleus.
Function:
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-, as well as 'Lys-63'-linked polyubiquitination. May be involved in degradation of muscle-specific proteins. Mediates polyubiquitination of CYP3A4.
Tissue Specificity:
Widely expressed, mainly in skeletal muscle.
Similarity:
Belongs to the ubiquitin-conjugating enzyme family.
SWISS:
P62253
Gene ID:
7326
Database links:Entrez Gene: 7326 Human
Entrez Gene: 67128 Mouse
Omim: 601569 Human
SwissProt: P62253 Human
SwissProt: P62254 Mouse
Unigene: 727591 Human
Unigene: 741319 Human
Unigene: 458052 Mouse
Product Picture Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Ube2G1) Polyclonal Antibody, Unconjugated (SL8371R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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