TEL: +86 571 56623320 EMAIL: [email protected]
Product Name Hi95 Chinese Name 缺氧诱导基因95抗体 Alias Hi95; Hypoxia induced gene 95; MGC11758; OTTHUMP00000003641; RP23 63D8.3; SES2; SESN 2; SEST2; Sestrin 2; DKFZp761M02121; OTTMUSP00000010184; DKFZp761M0212; SESN2_HUMAN. Research Area Cardiovascular Cell biology Signal transduction Immunogen Species Rabbit Clonality Polyclonal React Species Human, (predicted: Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=2ug/Test IF=1:50-200 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 54kDa Cellular localization The nucleus cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human SESN2/Hi95: 101-200/480 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail SESN2 is a member of the sestrin family of PA26-related proteins. The It may function in the regulation of cell growth and survival and also in cellular response to different stress conditions.
Function:
Involved in the reduction of peroxiredoxins.
Subcellular Location:
Cytoplasmic and Nuclear
Tissue Specificity:
Widely expressed.
Similarity:
Belongs to the sestrin family.
SWISS:
P58004
Gene ID:
83667
Database links:Entrez Gene: 83667 Human
Entrez Gene: 230784 Mouse
Omim: 607767 Human
SwissProt: P58004 Human
SwissProt: P58043 Mouse
Unigene: 469543 Human
Unigene: 23608 Mouse
Unigene: 37672 Rat
Product Picture Blank control:K562.
Primary Antibody (green line): Rabbit Anti-Hi95 antibody (SL8326R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.Blank control: K562.
Primary Antibody (green line): Rabbit Anti-Hi95 antibody (SL8326R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Scan Wechat Qrcode