Product Name	TEF4
Chinese Name	转录增强因子TEF4抗体
Alias	Embryonic TEA domain containing factor; Etdf; ETEF 1; ETF; TEA domain family member 2; TEAD 2; TEAD-2; TEAD2; Tead2 protein (TEA domain family member 2 (Mapped), isoform CRA_b); TEAD2_HUMAN; TEF 4; TEF4; Transcriptional enhancer factor 4; Transcriptional enhancer factor TEF 4; Transcriptional enhancer factor TEF-4.
Research Area	Cell biology  immunology  transcriptional regulatory factor  Epigenetics
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Mouse,  (predicted: Human, Rat, Dog, Cow, Horse, Rabbit, )
Applications	ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	49kDa
Cellular localization	The nucleus
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human TEA domain family member 2: 235-340/447
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	TEF-4 is strongly coexpressed with TEF-1 in mouse mitotic neuroblasts and is also detected in the gut and the nephrogenic region of the kidney. TEF-4 associates with the powerful transcriptional coactivator YAP65 to mediate mitogenic signals. In addition, TEF-4 promotes the activation of the CTP:phosphocholine cytidylyltransferase (CCT) alpha protein, which is the rate-limiting enzyme of phosphatidylcholine biosynthesis, by enhancing the transcriptional activity of Ets-1. Function: Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds to the SPH and GT-IIC 'enhansons' (5'-GTGGAATGT-3'). May be involved in the gene regulation of neural development. Binds to the M-CAT motif. Subunit: Interacts with YAP1 and WWTR1/TAZ. Subcellular Location: Nucleus. Similarity: Contains 1 TEA DNA-binding domain. SWISS: Q15562 Gene ID: 8463 Database links: Entrez Gene: 8463 Human Entrez Gene: 21677 Mouse Entrez Gene: 308582 Rat Omim: 601729 Human SwissProt: Q15562 Human SwissProt: P48301 Mouse Unigene: 515534 Human Unigene: 3019 Mouse Unigene: 130442 Rat
Product Picture	Sample: Lane 1: Mouse Cerebrum tissue lysates Lane 2: Rat Cerebrum tissue lysates Primary: Anti-TEF4 (SL8212R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 49 kDa Observed band size: 49 kDa Paraformaldehyde-fixed, paraffin embedded (mouse skin); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TEF4) Polyclonal Antibody, Unconjugated (SL8212R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TEF4) Polyclonal Antibody, Unconjugated (SL8212R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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