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Product Name FAM40A Chinese Name FAM家族蛋白40A抗体 Alias FA40A_HUMAN; fam40a; FLJ14743; KIAA1761; MGC148091; Protein FAM40A; RP4-773N10.1. Research Area Cell biology immunology Immunogen Species Rabbit Clonality Polyclonal React Species Human, (predicted: Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 96kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human FAM40A: 255-360/837 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Belongs to the FAM40 family. 4 isoforms of the human protein are produced by alternative splicing.
Function:
Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the cortical actin filament dynamics and cell shape.
Subcellular Location:
Cytoplasm.
Similarity:
Belongs to the FAM40 family.
SWISS:
Q5VSL9
Gene ID:
85369
Database links:
Entrez Gene: 85369 Human
SwissProt: Q5VSL9 Human
Unigene: 584996 Human
Unigene: 136895 Rat
Product Picture Sample:
Hela(Human) Cell Lysate at 30 ug
Primary: Anti-FAM40A (SL8194R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 96 kD
Observed band size: 96 kD
Tissue/cell: human brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-FAM40A Polyclonal Antibody, Unconjugated(SL8194R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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