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Product Name CCDC82 Chinese Name 卷曲螺旋结构域蛋白82抗体 Alias Coiled coil domain containing 82; coiled-coil domain containing 82; FLJ23518; HSPC048; HSPC048 protein; HT025; CCD82_HUMAN. Research Area Cell biology immunology Signal transduction Immunogen Species Rabbit Clonality Polyclonal React Species Rat, (predicted: Human, Mouse, Dog, Pig, Horse, ) Applications ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 64kDa Cellular localization The nucleus cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from Human CCDC82: 301-400/544 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail CCDC82 is an uncharacterized protein that bears a coiled-coil domain, a secondary structure composed of two or more alpha helices that functions as a helical cable.
Post-translational modifications:
Phosphorylated upon DNA damage, probably by ATM or ATR.
SWISS:
Q8N4S0
Gene ID:
79780
Database links:
UniProtKB/Swiss-Prot: Q8N4S0.2
Product Picture Tissue/cell: Rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CCDC82 Polyclonal Antibody, Unconjugated(SL8085R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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