Product Name	ADCK4
Chinese Name	ADCK4蛋白抗体
Alias	aarF domain containing protein kinase 4; Uncharacterized aarF domain containing protein kinase 4; ADCK4_HUMAN.
Research Area	Cell biology  Signal transduction  Kinases and Phosphatases
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Cow, Horse, Rabbit, )
Applications	ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:200-800 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	60kDa
Cellular localization	cytoplasmic The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human ADCK4: 65-170/544
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	ADCK4, AarF domain containing kinase 5, is a member of group i collagen (fibrillar forming collagen). The function of this protein is not yet clear. It is not known if it has protein kinase activity and what type of substrate it would phosphorylate (Ser, Thr or Tyr). There are 2 named isoforms produced by alternative splicing. Function: The function of this protein is not yet clear. It is notknown if it has protein kinase activity and what type of substrateit would phosphorylate (Ser, Thr or Tyr). Subcellular Location: Membrane; Single-pass membrane protein(Potential). Similarity: Belongs to the protein kinase superfamily. ADCKprotein kinase family. Contains 1 protein kinase domain. SWISS: Q96D53 Gene ID: 79934 Database links: Entrez Gene: 79934 Human Entrez Gene: 76889 Mouse Entrez Gene: 308453 Rat Omim: 615567 Human SwissProt: Q96D53 Human SwissProt: Q566J8 Mouse SwissProt: Q6AY19 Rat Unigene: 130712 Human Unigene: 124728 Mouse Unigene: 11549 Rat
Product Picture	Tissue/cell: HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (ADCK4) Polyclonal Antibody, Unconjugated (SL8070R) 1:200, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody (SL0295G-FITC) at 37°C for 90 minutes, DAPI (5ug/ml, blue, C-0033) was used to stain the cell nuclei. Paraformaldehyde-fixed, paraffin embedded (Rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ADCK4) Polyclonal Antibody, Unconjugated (SL8070R) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (SL0295G-FITC) for 90 minutes, and DAPI for nuclei staining. Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ADCK4) Polyclonal Antibody, Unconjugated (SL8070R) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (SL0295G-FITC) for 90 minutes, and DAPI for nuclei staining.

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