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Product Name ICMT Chinese Name 异戊烯半胱氨酸羧基甲基转移酶抗体 Alias CMT; HSTE14; Icmt; ICMT_HUMAN; Isoprenylcysteine carboxylmethyltransferase; MGC39955; MGC95322; MST098; MSTP098; pcCMT; PCMT; PPMT; Prenylated protein carboxyl methyltransferase; Prenylcysteine carboxyl methyltransferase; Protein-S-isoprenylcysteine O-methyltransferase; RP23-421E12.4; STE14; fcmt. Research Area Tumour Cell biology immunology transcriptional regulatory factor Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, (predicted: Rat, Dog, Pig, Cow, Sheep, ) Applications ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 32kDa Cellular localization cytoplasmic The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human CMT/Icmt: 211-278/284 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Catalyzes the post-translational methylation of isoprenylated C-terminal cysteine residues. Tissue specificity: Ubiquitously expressed. Expressed at higher levels in the cerebellum and putamen than in other brain regions. Abundant expression seen in the Purkinje cells and pontine neurons.
Function:
Catalyzes the post-translational methylation of isoprenylated C-terminal cysteine residues.
Subcellular Location:
Endoplasmic reticulum membrane.
Tissue Specificity:
Ubiquitously expressed. Expressed at higher levels in the cerebellum and putamen than in other brain regions. Abundant expression seen in the Purkinje cells and pontine neurons.
Similarity:
Belongs to the isoprenylcysteine O-methyltransferase family.
SWISS:
O60725
Gene ID:
23463
Database links:Entrez Gene: 23463 Human
Entrez Gene: 57295 Mouse
Omim: 605851 Human
SwissProt: O60725 Human
SwissProt: Q9EQK7 Mouse
Unigene: 515688 Human
Unigene: 277464 Mouse
Unigene: 48709 Rat
Icmt酶是异戊烯途径中的一种关键酶,它能将甲基加到Ras上。这种甲基标签能使Ras到达细胞中的最终目的地,Icmt酶能发送不控制生长的信号,因此,Icmt蛋白在保持细胞中的致癌基因Ras的开启状态中起到重要作用。(Ras是细胞的一种正常的遗传成分,但是这种基因的突变能够致使它促进细胞的失控生长。而且,在一半的结肠癌和90%的胰腺癌中发生了Ras基因突变)。Product Picture Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ICMT) Polyclonal Antibody, Unconjugated (SL8019R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Tissue/cell: human pancreatic carcinoma(BXPC-3);4% Paraformaldehyde-fixed;
Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-ICMT Polyclonal Antibody, Unconjugated(SL8019R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy5 conjugated(SL0295G-Cy5)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei
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