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Product Name SHOC2 Chinese Name 富含亮氨酸重复蛋白SHOC2抗体 Alias Leucine-rich repeat protein SHOC-2; Leucine-rich repeat protein SHOC-2; Ras-binding protein Sur-8; KIAA0862; Leucine-rich repeat protein SHOC-2; Protein soc-2 homolog; Protein Sur-8 homolog; SHOC2; SHOC2_HUMAN. Research Area Cell biology Signal transduction Cyclin Kinases and Phosphatases Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, Rat, (predicted: Chicken, Dog, Cow, Sheep, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 65kDa Cellular localization The nucleus cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human SHOC2/Sur8: 36-120/582 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Regulatory subunit of protein phosphatase 1 (PP1c) that acts as a M-Ras/MRAS effector and participates in MAPK pathway activation. Upon M-Ras/MRAS activation, targets PP1c to specifically dephosphorylate the 'Ser-259' inhibitory site of RAF1 kinase and stimulate RAF1 activity at specialized signaling complexes.
Involvement in disease:Defects in SHOC2 are the cause of Noonan syndrome-like with loose anagen hair (NSLAH) . NSLAH children display macrocephaly, high forehead, hypertelorism, palpebral ptosis, low-set and posteriorly rotated ears, short and webbed neck and pectus anomalies. Affected subjects also have easily pluckable, sparse, thin and slow-growing hair.
Function:
Regulatory subunit of protein phosphatase 1 (PP1c) that acts as a M-Ras/MRAS effector and participates in MAPK pathway activation. Upon M-Ras/MRAS activation, targets PP1c to specifically dephosphorylate the 'Ser-259' inhibitory site of RAF1 kinase and stimulate RAF1 activity at specialized signaling complexes.
Subunit:
Interacts with M-Ras/MRAS, and RAF1. Forms a multiprotein complex with Ras (M-Ras/MRAS), Raf (RAF1) and protein phosphatase 1 (PPP1CA, PPP1CB and PPP1CC). Interacts with ERBB2IP; disrupts the interaction with RAF1 and Ras, leading to prevent activation of the Ras signaling pathway. Specifically binds K-Ras/KRAS, M-Ras/MRAS and N-Ras/NRAS but not H-Ras/HRAS.
Subcellular Location:
Cytoplasm. Nucleus. Note=Translocates from cytoplasm to nucleus upon growth factor stimulation.
DISEASE:
Defects in SHOC2 are the cause of Noonan syndrome-like disorder with loose anagen hair (NSLH) [MIM:607721]. A syndrome characterized by Noonan dysmorphic features such as macrocephaly, high forehead, hypertelorism, palpebral ptosis, low-set and posteriorly rotated ears, short and webbed neck, pectus anomalies, in association with pluckable, sparse, thin and slow-growing hair.
Similarity:
Belongs to the SHOC2 family.
Contains 20 LRR (leucine-rich) repeats.
SWISS:
Q9UQ13
Gene ID:
8036
Database links:Entrez Gene: 8036 Human
Entrez Gene: 56392 Mouse
Omim: 602775 Human
SwissProt: Q9UQ13 Human
SwissProt: O88520 Mouse
Product Picture Sample:
Lane 1: Cerebrum (Mouse) Lysate at 40 ug
Lane 2: Testis (Mouse) Lysate at 40 ug
Lane 3: Esophagus (Mouse) Lysate at 40 ug
Lane 4: Large intestine (Mouse) Lysate at 40 ug
Lane 5: NIH/3T3 (Mouse) Cell Lysate at 30 ug
Lane 6: Cerebrum (Rat) Lysate at 40 ug
Lane 7: Testis (Rat) Lysate at 40 ug
Lane 8: Esophagus (Rat) Lysate at 40 ug
Lane 9: Large intestine (Rat) Lysate at 40 ug
Lane 10: A549 (Human) Cell Lysate at 30 ug
Lane 11: U937 (Human) Cell Lysate at 30 ug
Lane 12: HL60 (Human) Cell Lysate at 30 ug
Primary:
Anti- SHOC2 (SL7934R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 62 kD
Observed band size: 60 kD
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