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Product Name Iroquois homeobox protein 3 Chinese Name Irx3蛋白抗体 Alias IRXB1; Homeodomain protein IRXB1; Iroquois homeobox protein 3; Iroquois-class homeodomain protein IRX-3; IRX 1; Irx3; IRX3_HUMAN; AI894186. Research Area Cell biology Neurobiology Cyclin Cell differentiation Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, Rat, (predicted: Human, Dog, Pig, Cow, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 52kDa Cellular localization The nucleus Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human Iroquois homeobox protein 3: 401-501/501 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Transcription factor. Involved in SHH-dependent neural patterning. Together with NKX2-2 and NKX6-1 acts to restrict the generation of motor neurons to the appropriate region of the neural tube. Belongs to the class I proteins of neuronal progenitor factors, which are repressed by SHH signals. Involved in the transcriptional repression of MNX1 in non-motor neuron cells.
Function:
Transcription factor. Involved in SHH-dependent neural patterning. Together with NKX2-2 and NKX6-1 acts to restrict the generation of motor neurons to the appropriate region of the neural tube. Belongs to the class I proteins of neuronal progenitor factors, which are repressed by SHH signals. Involved in the transcriptional repression of MNX1 in non-motor neuron cells (By similarity).
Subunit:
Belongs to the TALE/IRO homeobox family.
Contains 1 homeobox DNA-binding domain.
Subcellular Location:
Nucleus.
Similarity:
Belongs to the TALE/IRO homeobox family.
Contains 1 homeobox DNA-binding domain.
SWISS:
P78415
Gene ID:
79191
Database links:
UniProtKB/Swiss-Prot: P78415.3Entrez Gene: 79191 Human
Entrez Gene: 16373 Mouse
Omim: 612985 Human
SwissProt: P78415 Human
SwissProt: P81067 Mouse
Product Picture Sample:
Heart (Mouse) Lysate at 40 ug
Primary: Anti- Iroquois homeobox protein 3 (SL7867R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kD
Observed band size: 53 kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Iroquois homeobox protein 3 Polyclonal Antibody, Unconjugated(SL7867R) 1:100, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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