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Product Name SKA3 Chinese Name 纺锤体和着丝粒相关蛋白3抗体 Alias C13orf3; RAMA1; SKA3; SKA3_HUMAN; Spindle and kinetochore associated complex subunit 3; Spindle and kinetochore-associated protein 3. literatures Specific References (2) | SL7848R has been referenced in 2 publications.[IF=5.959] Hou Y et al. SKA3 Promotes tumor growth by regulating CDK2/P53 phosphorylation in hepatocellular carcinoma. Cell Death Dis. 2019 Dec 5;10(12):929. WB&IHC-P ; Human.[IF=2.538] Haoyue Xu. et al. SKA3 promotes cell growth via the PI3K/AKT/GSK3β and PI3K/AKT/FOXO1 pathways and is a potential prognostic biomarker for oral squamous cell carcinoma. OR SURG OR MED OR PA. 2022 Jun;: WB, IHC ; Human, Mouse.Research Area Cell biology Cyclin Cell differentiation Immunogen Species Rabbit Clonality Polyclonal React Species Rat, (predicted: Human, Mouse, Chicken, Cow, Horse, Rabbit, Sheep, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 46kDa Cellular localization The nucleus cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human SKA3: 201-300/412 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Component of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies. The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner. In the complex, it mediates the microtubule-stimulated oligomerization.
Function:
Component of the SKA1 complex, a microtubule-bindingsubcomplex of the outer kinetochore that is essential for properchromosome segregation. The SKA1 complex is a direct component ofthe kinetochore-microtubule interface and directly associates withmicrotubules as oligomeric assemblies. The complex facilitates theprocessive movement of microspheres along a microtubule in adepolymerization-coupled manner. In the complex, it mediates themicrotubule-stimulated oligomerization.
Subunit:
Component of the SKA1 complex, composed of SKA1, SKA2 andSKA3. The core SKA1 complex is composed of 2 SKA1-SKA2heterodimers, each heterodimer interacting with a molecule of theSKA3 homodimer. The core SKA1 complex associates with microtubulesand forms oligomeric assemblies. Interacts directly with SKA1.
Subcellular Location:
Cytoplasm, cytoskeleton, spindle.Chromosome, centromere, kinetochore. Note=Localizes to the outerkinetochore and spindle microtubules during mitosis in a NDC80complex-dependent manner.
Similarity:
Belongs to the SKA3 family.
SWISS:
Q8IX90
Gene ID:
221150
Database links:Entrez Gene: 221150 Human
Entrez Gene: 219114 Mouse
SwissProt: Q8IX90 Human
SwissProt: Q8C263 Mouse
Unigene: 88523 Human
Unigene: 30173 Mouse
Unigene: 218204 Rat
Product Picture Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SKA3) Polyclonal Antibody, Unconjugated (SL7848R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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